ArticleParasites & vectors2015
Identification of phlebotomine sand fly blood meals by real-time PCR.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Identification of infection by Leishmania spp. in wild and domestic animals in Brazil: a systematic review with meta-analysis (2001-2021).Parasitology research · 2023Pooled it
- Processing and mounting phlebotomine sand flies: a consensus guideline.Parasite (Paris, France) · 2026Article
- A novel 3R-compliant ex vivo approach to assess ectoparasiticide repellency against Phlebotomus perniciosus in dogs.Parasite (Paris, France) · 2026Article
- Review
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- Bloodthirsty bites: host-feeding patterns of phlebotomine sand flies from two localities in the Aegean Region of Türkiye.BMC veterinary research · 2025Article
- Investigation ofPathogens (Basel, Switzerland) · 2025Article
- First Record ofPathogens (Basel, Switzerland) · 2025Article
- The complexities of blood-feeding patterns in mosquitoes and sandflies and the burden of disease: A minireview.Veterinary medicine and science · 2024Review
- Comparison of collection methods for Phlebotomus argentipes sand flies to use in a molecular xenomonitoring system for the surveillance of visceral leishmaniasis.PLoS neglected tropical diseases · 2023Article
- Efficiency of mitochondrial genes and nuclear Alu elements in detecting human DNA in blood meals of Anopheles stephensi mosquitoes: a time-course study.Parasites & vectors · 2023Article
- Investigating potential sand fly vectors after the first reported outbreak of cutaneous leishmaniasis in Ghana.Parasites & vectors · 2023Article
- Blood Meal Analysis and Molecular Detection of Leishmania DNA in Wild-Caught Sand Flies in Leishmaniasis Endemic Areas of Turkey and Northern Cyprus.Acta parasitologica · 2022Article
- Study on the zoonotic cycle of tegumentary leishmaniasis in an endemic area of a metropolitan region in the Northeastern region of Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- First molecular evidence of frogs as a food source for sand flies (Diptera: Phlebotominae) in Brazilian caves.Parasitology research · 2021Article
- Lutzomyia longipalpis, Gone with the Wind and Other Variables.Neotropical entomology · 2021Review
- Review
- Evaluation of different storage times and preservation methods on phlebotomine sand fly DNA concentration and purity.Parasites & vectors · 2020Article
- Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies.Parasites & vectors · 2020Article
- Feeding behavior and activity of Phlebotomus pedifer and potential reservoir hosts of Leishmania aethiopica in southwestern Ethiopia.PLoS neglected tropical diseases · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhlebotomine sand flies are blood-feeding insects of great medical and veterinary significance acting as vectors of Leishmania parasites. Studying the blood-feeding pattern of these insects may help in the understanding of their interactions with potential reservoir hosts of Leishmania parasites. In this study, we developed real time PCR assays for the identification of sand fly blood meal.
methodsSix pairs of primers were designed based on cytochrome b gene sequences available in GenBank of the following potential hosts: dog, cat, horse, chicken, black rat, and human. Firstly, SYBR Green-based real time PCR assays were conducted using a standard curve with eight different concentrations (i.e., 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg and 1 fg per 2 μl) of DNA samples extracted from EDTA blood samples from each target animal. Then, DNA samples extracted from field-collected engorged female sand flies belonging to three species (i.e., Lutzomyia longipalpis, L. migonei and L. lenti) were tested by the protocols standardized herein. Additionally, female sand flies were experimentally fed on a black rat (Rattus rattus) and used for evaluating the time course of the detection of the protocol targeting this species.
resultsThe protocols performed well with detection limits of 10 pg to 100 fg. Field-collected female sand flies were fed on blood from humans (73%), chickens (23%), dogs (22%), horses (15%), black rats (11%) and cats (2%). Interestingly, 76.1% of the L. longipalpis females were positive for human blood. In total, 48% of the tested females were fed on single sources, 31% on two and 12% on three. The analysis of the time course showed that the real time PCR protocol targeting the black rat DNA was able to detect small amounts of the host DNA up to 5 days after the blood meal.
conclusionsThe real time PCR assays standardized herein successfully detected small amounts of host DNA in female sand flies fed on different vertebrate species and, specifically for the black rats, up to 5 days after the blood meal. These assays represent promising tools for the identification of blood meal in field-collected female sand flies.
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