ArticleReproductive biology and endocrinology : RB&E2015
MicroRNA-181a is involved in the regulation of human endometrial stromal cell decidualization by inhibiting Krüppel-like factor 12.
Article in Reproductive biology and endocrinology : RB&E, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 52 citations in OpenAlex.
- M2 Microglia-Derived Exosomal miR-144-5p Attenuates White Matter Injury in Preterm Infants by Regulating the PTEN/AKT Pathway Through KLF12.Molecular biotechnology · 2026Article
- MicroRNAs, endometrial receptivity and molecular pathways.Reproductive biology and endocrinology : RB&E · 2024Review
- The role of viral infection in implantation failure: direct and indirect effects.Reproductive biology and endocrinology : RB&E · 2024Review
- When the Embryo Meets the Endometrium: Identifying the Features Required for Successful Embryo Implantation.International journal of molecular sciences · 2024Review
- MiR-124-3p negatively impacts embryo implantation via suppressing uterine receptivity formation and embryo development.Reproductive biology and endocrinology : RB&E · 2024Article
- Identification of circulatory microRNA based biomarkers for early pregnancy diagnosis in buffalo.Frontiers in cell and developmental biology · 2024Article
- The Pathological Role of miRNAs in Endometriosis.Biomedicines · 2023Review
- Circulating miRNAs as a Tool for Early Diagnosis of Endometrial Cancer-Implications for the Fertility-Sparing Process: Clinical, Biological, and Legal Aspects.International journal of molecular sciences · 2023Review
- MicroRNAs, small regulatory elements with significant effects on human implantation: a review.Journal of assisted reproduction and genetics · 2023Review
- Impairment of Decidualization of Endometrial Stromal Cells by hsa-miR-375 Through NOX4 Targeting.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
- MicroRNAs in the Regulation of Endometrial Receptivity for Embryo Implantation.International journal of molecular sciences · 2022Review
- Role of endometrial microRNAs in repeated implantation failure (mini-review).Frontiers in cell and developmental biology · 2022Review
- Non-Invasive and Mechanism-Based Molecular Assessment of Endometrial Receptivity During the Window of Implantation: Current Concepts and Future Prospective Testing Directions.Frontiers in reproductive health · 2022Review
- miRNAs in decidual NK cells: regulators worthy of attention during pregnancy.Reproductive biology and endocrinology : RB&E · 2021Review
- MiR-148a-3p may contribute to flawed decidualization in recurrent implantation failure by modulating HOXC8.Journal of assisted reproduction and genetics · 2020Article
- Endometrial Decidualization: The Primary Driver of Pregnancy Health.International journal of molecular sciences · 2020Review
- Epigenetic modifications working in the decidualization and endometrial receptivity.Cellular and molecular life sciences : CMLS · 2020Review
- Physiologic Events of Embryo Implantation and Decidualization in Human and Non-Human Primates.International journal of molecular sciences · 2020Review
- Fetal Membrane Epigenetics.Frontiers in physiology · 2020Review
- Secreted MicroRNA to Predict Embryo Implantation Outcome: From Research to Clinical Diagnostic Application.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe transformation of endometrium into decidua is essential for normal implantation of the blastocyst. However, the post-transcriptional regulation and the miRNAs involved in decidualization remain poorly understood. Here, we examined microRNA-181a (miR-181a) expression in decidualized human endometrial stromal cell (hESC). In addition, we investigated the functional effect of miR-181a on hESC decidualization in vitro.
methodsQuantitative real-time PCR (qRT-PCR) was used to detect the profile of miR-181a in decidualized hESC. qRT-PCR, enzyme-linked fluorescent assay, and immunofluorescence assay were performed to investigate decidualization marker genes' expression after enhancing or inhibition of miR-181a expression in hESC. Luciferase reporter assay, western blotting, qRT-PCR, and immunofluorescence assay were carried out to identify the relationship between miR-181a and Krüppel-like factor 12 (KLF12).
resultsmiR-181a expression levels increased dramatically in hESC treated with 8-Br-cAMP and MPA. Increased miR-181a expression promoted hESC decidualization-related gene expression and morphological transformation; conversely, inhibition of miR-181a expression compromised hESC decidualization in vitro. Further analysis confirmed that miR-181a interacted with the 3' untranslated region of the transcription factor KLF12 and down-regulated KLF12 at the transcriptional and translational levels. KLF12 overexpression abolished miR-181a-induced decidualization.
conclusionsOur findings suggest that miR-181a plays a functionally important role in human endometrial stromal cell decidualization in vitro by inhibiting KLF12.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.