ArticleParasites & vectors2015
Serological differentiation of antibodies against Rickettsia helvetica, R. raoultii, R. slovaca, R. monacensis and R. felis in dogs from Germany by a micro-immunofluorescent antibody test.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Simultaneous serological assessment of four zoonotic rickettsiae among dogs near the United States-Mexico border.PLoS neglected tropical diseases · 2026Article
- Rickettsia spp. in finnish ixodid ticks.Parasites & vectors · 2025Article
- Prevalence of tick-borne bacterial pathogens in Germany-has the situation changed after a decade?Frontiers in cellular and infection microbiology · 2024Article
- Molecular Survey ofVeterinary sciences · 2023Article
- Rickettsial Infection in the COVID-19 Era: The Correlation between the Detection ofMicroorganisms · 2023Article
- Revealing the Tick Microbiome: Insights into Midgut and Salivary Gland Microbiota of FemaleInternational journal of molecular sciences · 2023Article
- Risk factors analysis for neglected human rickettsioses in rural communities in Nan province, Thailand: A community-based observational study along a landscape gradient.PLoS neglected tropical diseases · 2022Observational
- Detection of Tick-Borne Pathogens of the GeneraPathogens (Basel, Switzerland) · 2021Article
- Preliminary Evidence ofPathogens (Basel, Switzerland) · 2021Article
- Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.Parasites & vectors · 2021Article
- Zoonotic Tick-Borne Pathogens in Temperate and Cold Regions of Europe-A Review on the Prevalence in Domestic Animals.Frontiers in veterinary science · 2020Review
- Tick-borne pathogens in ticks collected from dogs, Latvia, 2011-2016.BMC veterinary research · 2019Article
- Combination of microbiome analysis and serodiagnostics to assess the risk of pathogen transmission by ticks to humans and animals in central Germany.Parasites & vectors · 2019Article
- Neglected aspects of tick-borne rickettsioses.Parasites & vectors · 2018Review
- The diversity of tick-borne bacteria and parasites in ticks collected from the Strandja Nature Park in south-eastern Bulgaria.Parasites & vectors · 2018Article
- First report of Rickettsia raoultii and R. slovaca in Melophagus ovinus, the sheep ked.Parasites & vectors · 2016Article
- The ecological niche of Dermacentor marginatus in Germany.Parasitology research · 2016Article
- Detection of vector-borne pathogens in cats and their ectoparasites in southern Italy.Parasites & vectors · 2016Article
- Pathogens vectored by the tick, Dermacentor reticulatus, in endemic regions and zones of expansion in Poland.Parasites & vectors · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSpotted Fever Group (SFG) Rickettsiae can cause febrile diseases with or without rash in humans worldwide. In Germany only limited data are available about their medical significance. Serological screening tests for antibodies against rickettsiae usually only distinguish between SFG and Typhus Group (TG) Rickettsiae due to the strong cross reactivities within the groups. Seroprevalence rates in dogs, as possible sentinels for tick-borne diseases, could serve as an indicator for the distribution of different Rickettsia species.
methodsIn this study, a micro-immunofluorescence assay (micro-IFA) was established for detection and differentiation of antibodies against five Rickettsia species in dogs (R. helvetica, R. raoultii, R. slovaca, R. monacensis and R. felis). Dogs that never left Germany (n = 605) previously investigated with an SFG-ELISA were included in this study and screened at a 1:128 dilution. Endpoint titres of fifty randomly selected seropositive samples of each of the five investigated regions in Germany were determined in order to allow a differentiation of the causative Rickettsia species. Sensitivity and specificity of the micro-IFA were compared with ELISA results of the previous study.
resultsA total of 93.9% of the dogs were positive for antibodies of the SFG Rickettsiae at the screening titer of 1:128. Differentiation of SFG Rickettsiae with the micro-IFA was possible in 70.4%, but in 29.6% of the cases the detected antibodies were not differentiable. Considering a clear differentiation by a twofold titre difference between observed reactions, the seroprevalence rates were 66.0% for R. helvetica, 2.8% for R. raoultii, 1.6% for R. slovaca, but no serological reaction could be clearly attributed to R. monacensis or R. felis. No statistically significant regional differences were found for R. helvetica, R. slovaca and R. raoultii comparing the five regions of Germany. Comparison of micro-IFA with ELISA revealed a sensitivity of 82.0% and a specificity of 83.8% for the Rickettsia SFG ELISA.
conclusionsThe micro-IFA is a useful serological tool to differentiate antibodies against different Rickettsia species in dogs. Seroprevalence rates in dogs correspond to the prevalence rates and distribution of Rickettsia-carrying tick species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.