ArticleMicrobial cell factories2015
Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 105 citations in OpenAlex.
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- Modular co-culture engineering of Yarrowia lipolytica for amorphadiene biosynthesis.Microbial cell factories · 2022Article
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- Enhancing fluxes through the mevalonate pathway in Saccharomyces cerevisiae by engineering the HMGR and β-alanine metabolism.Microbial biotechnology · 2022Article
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- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Dual regulation of lipid droplet-triacylglycerol metabolism and ERG9 expression for improved β-carotene production in Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- CRISPRi-Guided Metabolic Flux Engineering for Enhanced Protopanaxadiol Production inInternational journal of molecular sciences · 2021Article
- Amorpha-4,11-diene synthase: a key enzyme in artemisinin biosynthesis and engineering.aBIOTECH · 2021Review
- Optogenetic Amplification Circuits for Light-Induced Metabolic Control.ACS synthetic biology · 2021Article
- Dynamic control in metabolic engineering: Theories, tools, and applications.Metabolic engineering · 2021Review
- Engineering a growth-phase-dependent biosynthetic pathway for carotenoid production in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2020Article
- Discovery and identification of medium-chain fatty acid responsive promoters inEngineering in life sciences · 2020Article
- Production of Terpenoids by Synthetic Biology Approaches.Frontiers in bioengineering and biotechnology · 2020Review
- Combining transcriptomics and metabolomics to reveal the underlying molecular mechanism of ergosterol biosynthesis during the fruiting process of Flammulina velutipes.BMC genomics · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo achieve high-level production of non-native isoprenoid products, it requires the metabolic flux to be diverted from the production of sterols to the heterologous metabolic reactions. However, there are limited tools for restricting metabolic flux towards ergosterol synthesis. In the present study, we explored dynamic control of ERG9 expression using different ergosterol-responsive promoters to improve the production of non-native isoprenoids.
resultsSeveral ergosterol-responsive promoters were identified using quantitative real-time PCR (qRT-PCR) analysis in an engineered strain with relatively high mevalonate pathway activity. We found mRNA levels for ERG11, ERG2 and ERG3 expression were significantly lower in the engineered strain over the reference strain BY4742, indicating these genes are transcriptionally down-regulated when ergosterol is in excess. Further replacement of the native ERG9 promoter with these ergosterol-responsive promoters revealed that all engineered strains improved amorpha-4,11-diene by 2~5-fold over the reference strain with ERG9 under its native promoter. The best engineered strain with ERG9 under the control of P ERG1 produced amorpha-4,11-diene to a titer around 350 mg/L after 96 h cultivation in shake-flasks.
conclusionsWe envision dynamic control at the branching step using feedback regulation at transcriptional level could serve as a generalized approach for redirecting the metabolic flux towards product-of-interest.
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