ArticleMicrobial cell factories2015
Metabolic network analysis and experimental study of lipid production in Rhodosporidium toruloides grown on single and mixed substrates.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 138 citations in OpenAlex.
- Engineering Rhodotorula toruloides as a platform organism for de novo synthesis of fatty-acid esters.Nature communications · 2026Article
- Effects of inorganic nitrogen addition to okara-utilized medium on the oleaginous yeast Lipomyces starkeyi and assessment of metabolism involved in increased oil production.Biotechnology for biofuels and bioproducts · 2025Article
- The Plasma Membrane H+-ATPase Promoter Driving the Expression ofJournal of fungi (Basel, Switzerland) · 2024Article
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Rhodotorula toruloides: an ideal microbial cell factory to produce oleochemicals, carotenoids, and other products.World journal of microbiology & biotechnology · 2021Review
- The history, state of the art and future prospects for oleaginous yeast research.Microbial cell factories · 2021Review
- A metabolic model of Lipomyces starkeyi for predicting lipogenesis potential from diverse low-cost substrates.Biotechnology for biofuels · 2021Article
- Transcriptomic and metabolomic profiling of a Rhodotorula color mutant to improve its lipid productivity in fed-batch fermentation.World journal of microbiology & biotechnology · 2021Article
- Evaluating accessibility, usability and interoperability of genome-scale metabolic models for diverse yeasts species.FEMS yeast research · 2021Article
- Mixed glycerol and orange peel-based substrate for fed-batch microbial biodiesel production.Heliyon · 2020Article
- Rhodosporidium toruloides - A potential red yeast chassis for lipids and beyond.FEMS yeast research · 2020Review
- Carotenoids and lipid production fromBiotechnology for biofuels · 2020Article
- Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization inFrontiers in bioengineering and biotechnology · 2020Article
- Xylose Metabolism and the Effect of Oxidative Stress on Lipid and Carotenoid Production inFrontiers in bioengineering and biotechnology · 2020Article
- Article
- Fast media optimization for mixotrophic cultivation of Chlorella vulgaris.Scientific reports · 2019Article
- A comprehensive genome-scale model forMetabolic engineering communications · 2019Article
- Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams.Foods (Basel, Switzerland) · 2019Article
- Improved electrocompetence and metabolic engineering ofEngineering in life sciences · 2019Article
- Co-utilization of acidified glycerol pretreated-sugarcane bagasse for microbial oil production by a novelEngineering in life sciences · 2019Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrobial lipids (triacylglycerols, TAG) have received large attention for a sustainable production of oleochemicals and biofuels. Rhodosporidium toruloides can accumulate lipids up to 70% of its cell mass under certain conditions. However, our understanding of lipid production in this yeast is still much limited, especially for growth with mixed substrates at the level of metabolic network. In this work, the potentials of several important carbon sources for TAG production in R.toruloides are first comparatively studied in silico by means of elementary mode analysis followed by experimental validation.
resultsA simplified metabolic network of R.toruloides was reconstructed based on a combination of genome and proteome annotations. Optimal metabolic space was studied using elementary mode analysis for growth on glycerol, glucose, xylose and arabinose or in mixtures. The in silico model predictions of growth and lipid production are in agreement with experimental results. Both the in silico and experimental studies revealed that glycerol is an attractive substrate for lipid synthesis in R. toruloides either alone or in blend with sugars. A lipid yield as high as 0.53 (C-mol TAG/C-mol) has been experimentally obtained for growth on glycerol, compared to a theoretical maximum of 0.63 (C-mol TAG/C-mol). The lipid yield on glucose is much lower (0.29 (experimental) vs. 0.58 (predicted) C-mol TAG/C-mol). The blend of glucose with glycerol decreased the lipid yield on substrate but can significantly increase the overall volumetric productivity. Experimental studies revealed catabolite repression of glycerol by the presence of glucose for the first time. Significant influence of oxygen concentration on the yield and composition of lipids were observed which have not been quantitatively studied before.
conclusionsThis study provides for the first time a simplified metabolic model of R.toruloides and its detailed in silico analysis for growth on different carbon sources for their potential of TAG synthesis. Experimental studies revealed the phenomenon of catabolite repression of glycerol by glucose and the importance of oxygen supply on the yield and composition of lipids. More systematic studies are needed to understand the mechanisms which should help to further optimize the lipid production in this strain of industrial interest.
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