ArticleReproductive biology and endocrinology : RB&E2015
Isoform 165 of vascular endothelial growth factor in collagen matrix improves ovine cryopreserved ovarian tissue revascularisation after xenotransplantation in mice.
Article in Reproductive biology and endocrinology : RB&E, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Ovarian puncture alters genes but not follicle growth in cyclophosphamide mice model.Reproduction & fertility · 2026Article
- The mechanism and protective strategies of follicle injury after ovarian tissue cryopreservation and thawed transplantation: a review.Journal of ovarian research · 2025Review
- Endothelial cell supplementation promotes xenograft revascularization during short-term ovarian tissue transplantation.Bioactive materials · 2025Article
- BEZ235-Mediated PI3K/mTOR dual inhibition improves ovarian follicle survival in a preclinical model.Reproductive biology and endocrinology : RB&E · 2025Article
- Thawing fertility: a view of ovarian tissue cryopreservation processes and review of ovarian transplant research.Fertility and sterility · 2024Review
- Review
- The mTOR Inhibitor Rapamycin Counteracts Follicle Activation Induced by Ovarian Cryopreservation in Murine Transplantation Models.Medicina (Kaunas, Lithuania) · 2023Article
- Age-related fertility decline: is there a role for elective ovarian tissue cryopreservation?Human reproduction (Oxford, England) · 2022Review
- Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF.Reproduction & fertility · 2021Article
- The challenge of ovarian tissue culture: 2D versus 3D culture.Journal of ovarian research · 2021Article
- Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture.Journal of ovarian research · 2021Article
- Hypothermic machine perfusion after static cold storage improves ovarian function in rat ovarian tissue transplantation.Journal of assisted reproduction and genetics · 2020Article
- Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF.PloS one · 2020Article
- Influence of graft size, histocompatibility,and cryopreservation on reproductive outcome following ovary transplantation in mice.Journal of assisted reproduction and genetics · 2019Article
- Evaluation of Z-VAD-FMK as an anti-apoptotic drug to prevent granulosa cell apoptosis and follicular death after human ovarian tissue transplantation.Journal of assisted reproduction and genetics · 2019Article
- Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival.Journal of ovarian research · 2016Article
- In vitro evaluation of the anti-apoptotic drug Z-VAD-FMK on human ovarian granulosa cell lines for further use in ovarian tissue transplantation.Journal of assisted reproduction and genetics · 2015Article
- Scaffold-based delivery of adipose tissue-derived stem cells in rat frozen-thawed ovarian autografts: preliminary studies in a rat model.Journal of assisted reproduction and genetics · 2015Article
- Influence of mouse strain on ovarian tissue recovery after engraftment with angiogenic factor.Journal of ovarian research · 2015Article
- Protective Effects of Reduced Glutathione and Ulinastatin on Xeno-transplanted Human Ovarian Tissue Against Ischemia and Reperfusion Injury.Cell transplantationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAggressive anti-cancer treatments can result in ovarian failure. Ovarian cryopreservation has been developed to preserve the fertility of young women, but early graft revascularisation still requires improvement.
methodsFrozen/thawed sheep ovarian cortical biopsies were embedded in collagen matrix with or without isoform 165 of vascular endothelial growth factor (VEGF165) and transplanted into ovaries of immunodeficient mice. Ovaries were chosen as transplantation sites to more closely resemble clinical conditions in which orthotopic transplantation has previously allowed several spontaneous pregnancies.
resultsWe found that VEGF165 significantly increased the number of Dextran-FITC positive functional vessels 3 days after grafting. Dextran- fluorescein isothiocyanate (FITC) positive vessels were detectable in 53% and 29% of the mice in the VEGF-treated and control groups, respectively. Among these positive fragments, 50% in the treated group displayed mature smooth-muscle-actin-alpha (alpha-SMA) positive functional vessels compared with 0% in the control group. CD31 positive murine blood vessels were observed in 40% of the VEGF165 transplants compared with 21% of the controls. After 3 weeks, the density of murine vessels was significantly higher in the VEGF165 group.
conclusionThe encapsulation of ovarian tissue in collagen matrix in the presence of VEGF165 before grafting has a positive effect on functional blood vessel recruitment. It can be considered as a useful technique to be improved and further developed before human clinical applications in female cancer patients in the context of fertility preservation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.