ArticleVeterinary research2015
Piscine orthoreovirus (PRV) replicates in Atlantic salmon (Salmo salar L.) erythrocytes ex vivo.
Article in Veterinary research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 105 citations in OpenAlex.
- Vaccination Reduces Shedding of Salmonid Alphavirus Subtype 3, but Bacterial Co-Infection Influences the Effect.Journal of fish diseases · 2026Article
- Transcriptome Analysis ofAnimals : an open access journal from MDPI · 2026Article
- Identification and characterization of a novel orthoreovirus from American eels,Microbiology spectrum · 2026Article
- Toward Effective Vaccines Against Piscine Orthoreovirus: Challenges and Current Strategies.Viruses · 2025Review
- Effects of glucocorticoid receptor activation on gene expression and antiviral responses in Atlantic salmon (Salmo salar L.) red blood cells.Veterinary research · 2025Article
- Comparative Analysis of PRV-1 in Atlantic Salmon and PRV-3 in Coho Salmon: Host-Specific Immune Responses and Apoptosis in Red Blood Cells.Microorganisms · 2025Article
- Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses.Journal of fish diseases · 2025Article
- Evaluating Atlantic Salmon (Pathogens (Basel, Switzerland) · 2024Article
- A novel high-throughput qPCR chip for solving co-infections in RAS farmed rainbow trout.Scientific reports · 2024Article
- Analyses and Insights into Genetic Reassortment and Natural Selection as Key Drivers ofViruses · 2024Article
- Cellular heterogeneity in red and melanized focal muscle changes in farmed Atlantic salmon (Salmo salar) visualized by spatial transcriptomics.Cell and tissue research · 2024Article
- Transcriptomics of early responses to purifiedFrontiers in immunology · 2024Article
- Long-term persistence of piscine orthoreovirus-1 (PRV-1) infection during the pre-smolt stages of Atlantic salmon in freshwater.Veterinary research · 2023Article
- Innate immune response of rainbow trout erythrocytes to spinycterins expressing a downsized viral fragment of viral haemorrhagic septicaemia virus.Aquaculture (Amsterdam, Netherlands) · 2023Article
- Fish Innate Immune Response to Viral Infection-An Overview of Five Major Antiviral Genes.Viruses · 2022Review
- Article
- Sleeping With the Enemy? The Current Knowledge of Piscine Orthoreovirus (PRV) Immune Response Elicited to Counteract Infection.Frontiers in immunology · 2022Review
- Type II Grass Carp Reovirus Infects Leukocytes but Not Erythrocytes and Thrombocytes in Grass Carp (Viruses · 2021Article
- Article
- Extensive Phylogenetic Analysis of Piscine Orthoreovirus Genomic Sequences Shows the Robustness of Subgenotype Classification.Pathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Piscine orthoreovirus (PRV) is a reovirus that has predominantly been detected in Atlantic salmon (Salmo salar L.). PRV is associated with heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon, and recently erythrocytes were identified as major target cells. The study of PRV replication and pathogenesis of the infection has been impeded by the inability to propagate PRV in vitro. In this study we developed an ex vivo cultivation system for PRV in Atlantic salmon erythrocytes. PRV was successfully passaged to naïve erythrocytes using lysates of blood cells from infected salmon. During cultivation a significant increase in viral load was observed by RT-qPCR and flow cytometry, which coincided with the formation of cytoplasmic inclusions. The inclusions resembled viral factories and contained both PRV protein and dsRNA. In addition, the erythrocytes generated an antiviral immune gene activation after PRV infection, with significant up-regulation of IFN-α, RIG-I, Mx and PKR transcripts. Supernatants from the first passage successfully transmitted virus to naïve erythrocytes. This study demonstrates that PRV replicates in Atlantic salmon erythrocytes ex vivo. The ex vivo infection model closely reflects the situation in vivo and can be used to study the infection and replication mechanisms of PRV, as well as the antiviral immune responses of salmonid erythrocytes.
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