ArticleBMC genomics2015
Full genome SNP-based phylogenetic analysis reveals the origin and global spread of Brucella melitensis.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- Brucellosis in Southeast Asia: A Systematic Review of Epidemiological Evidence, Surveillance Gaps, and cgMLST-Based Insights Into Genetic Relatedness.Transboundary and emerging diseases · 2026Pooled it
- Genomic Epidemiology and Antimicrobial Susceptibility of Human Brucellosis - Shenzhen City, Guangdong Province, China, 2005-2024.China CDC weekly · 2026Article
- Investigation of Possible Intraoperative Transmission of Brucella melitensis, Slovenia.Emerging infectious diseases · 2025Article
- Genome phylogenetic analysis of Brucella melitensis in Northwest China.BMC microbiology · 2025Article
- Genomic analysis of Brucella melitensis isolates recovered from humans in south Tunisia over 35 years between 1988 and 2022.BMC microbiology · 2025Article
- Global Species/Biovars and Genotype Diversity Atlas ofChina CDC weekly · 2025Article
- Whole-genome sequencing-based analysis of Brucella species isolated from ruminants in various regions of Türki̇ye.BMC infectious diseases · 2024Article
- Genomic analysis ofEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Analysis of the Brucella melitensis epidemic in Xinjiang: genotyping, polymorphism, antibiotic resistance and tracing.Annals of clinical microbiology and antimicrobials · 2024Article
- Molecular typing methods to characterizeVeterinary world · 2024Review
- Phylogenetic Analysis and Comparative Genomics of Brucella abortus and Brucella melitensis Strains in Egypt.Journal of molecular evolution · 2024Article
- Comparative genomics of Brucella abortus and Brucella melitensis unravels the gene sharing, virulence factors and SNP diversity among the standard, vaccine and field strains.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Non-classicalThe Journal of international medical research · 2024Article
- Exploring genetic determinants of antimicrobial resistance inFrontiers in microbiology · 2024Article
- Strong Homology Between Colonizing and Bloodstream Carbapenem-ResistantInfection and drug resistance · 2024Article
- Genotypic peculiarities of a human brucellosis case caused by Brucella suis biovar 5.Scientific reports · 2023Article
- Article
- Genotype diversity of brucellosis agents isolated from humans and animals in Greece based on whole-genome sequencing.BMC infectious diseases · 2023Article
- A 14th century CE Brucella melitensis genome and the recent expansion of the Western Mediterranean clade.PLoS pathogens · 2023Article
- Whole-genome sequencing for genetic diversity analysis of IranianOne health (Amsterdam, Netherlands) · 2023Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBrucellosis is an important zoonotic disease that affects both humans and animals. We sequenced the full genome and characterised the genetic diversity of two Brucella melitensis isolates from Malaysia and the Philippines. In addition, we performed a comparative whole-genome single nucleotide polymorphism (SNP) analysis of B. melitensis strains collected from around the world, to investigate the potential origin and the history of the global spread of B. melitensis.
resultsSingle sequencing runs of each genome resulted in draft genome sequences of MY1483/09 and Phil1136/12, which covered 99.85% and 99.92% of the complete genome sequences, respectively. The B. melitensis genome sequences, and two B. abortus strains used as the outgroup strains, yielded a total of 13,728 SNP sites. Phylogenetic analysis using whole-genome SNPs and geographical distribution of the isolates revealed spatial clustering of the B. melitensis isolates into five genotypes, I, II, III, IV and V. The Mediterranean strains, identified as genotype I, occupied the basal node of the phylogenetic tree, suggesting that B. melitensis may have originated from the Mediterranean regions. All of the Asian B. melitensis strains clustered into genotype II with the SEA strains, including the two isolates sequenced in this study, forming a distinct clade denoted here as genotype IId. Genotypes III, IV and V of B. melitensis demonstrated a restricted geographical distribution, with genotype III representing the African lineage, genotype IV representing the European lineage and genotype V representing the American lineage.
conclusionWe showed that SNPs retrieved from the B. melitensis draft full genomes were sufficient to resolve the interspecies relationships between B. melitensis strains and to discriminate between the vaccine and endemic strains. Phylogeographic reconstruction of the history of B. melitensis global spread at a finer scale by using whole-genome SNP analyses supported the origin of all B. melitensis strains from the Mediterranean region. The possible global distribution of B. melitensis following the ancient trade routes was also consistent with whole-genome SNP phylogeny. The whole genome SNP phylogenetics analysis, hence is a powerful tool for intraspecies discrimination of closely related species.
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