ArticleBMC genomics2015
Identification of three extra-chromosomal replicons in Leptospira pathogenic strain and development of new shuttle vectors.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Leptospira and Leptospirosis: A Review of Species Classifications, Genomes, Morphological Structures, Antimicrobial Resistances, Transmissions, and Clinical Manifestations.Current microbiology · 2026Review
- Leptospira and Leptospirosis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Genomic Relationship BetweenGenes · 2025Article
- ExploringMicroorganisms · 2024Article
- Current treatment options for leptospirosis: a mini-review.Frontiers in microbiology · 2024Review
- Chronic Release of Tailless Phage Particles from Lactococcus lactis.Applied and environmental microbiology · 2022Article
- Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages.mBio · 2020Article
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- Characterization of LE3 and LE4, the only lytic phages known to infect the spirochete Leptospira.Scientific reports · 2018Article
- Function, expression, specificity, diversity and incompatibility of actinobacteriophage parABS systems.Molecular microbiology · 2016Article
- Distribution of Plasmids in Distinct Leptospira Pathogenic Species.PLoS neglected tropical diseases · 2015Article
- Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genome of pathogenic Leptospira interrogans contains two chromosomes. Plasmids and prophages are known to play specific roles in gene transfer in bacteria and can potentially serve as efficient genetic tools in these organisms. Although plasmids and prophage remnants have recently been reported in Leptospira species, their characteristics and potential applications in leptospiral genetic transformation systems have not been fully evaluated.
resultsThree extrachromosomal replicons designated lcp1 (65,732 bp), lcp2 (56,757 bp), and lcp3 (54,986 bp) in the L. interrogans serovar Linhai strain 56609 were identified through whole genome sequencing. All three replicons were stable outside of the bacterial chromosomes. Phage particles were observed in the culture supernatant of 56609 after mitomycin C induction, and lcp3, which contained phage-related genes, was considered to be an inducible prophage. L. interrogans-Escherichia coli shuttle vectors, constructed with the predicted replication elements of single rep or rep combined with parAB loci from the three plasmids were shown to successfully transform into both saprophytic and pathogenic Leptospira species, suggesting an essential function for rep genes in supporting auto-replication of the plasmids. Additionally, a wide distribution of homologs of the three rep genes was identified in L. interrogans isolates, and correlation tests showed that the transformability of the shuttle vectors in L. interrogans isolates depended, to certain extent, on genetic compatibility between the rep sequences of both plasmid and host.
conclusionsThree extrachromosomal replicons co-exist in L. interrogans, one of which we consider to be an inducible prophage. The vectors constructed with the rep genes of the three replicons successfully transformed into saprophytic and pathogenic Leptospira species alike, but this was partly dependent on genetic compatibility between the rep sequences of both plasmid and host.
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