ArticleMolecular neurodegeneration2015
An automated image analysis method to measure regularity in biological patterns: a case study in a Drosophila neurodegenerative model.
Article in Molecular neurodegeneration, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Low Angle Ring Illumination Stereomicroscopy (LARIS) Method for High-Contrast Imaging ofBio-protocol · 2026Article
- Brazilian kefir fraction mitigates the Alzheimer-like phenotype in Drosophila melanogaster with β-amyloid overexpression model.Scientific reports · 2024Article
- An accessible digital imaging workflow for multiplexed quantitative analysis of adult eye phenotypes inbioRxiv : the preprint server for biology · 2024Article
- Increase in brain glycogen levels ameliorates Huntington's disease phenotype and rescues neurodegeneration in Drosophila.Disease models & mechanisms · 2023Article
- Using Drosophila to identify naturally occurring genetic modifiers of amyloid beta 42- and tau-induced toxicity.G3 (Bethesda, Md.) · 2023Article
- SRSF1 haploinsufficiency is responsible for a syndromic developmental disorder associated with intellectual disability.American journal of human genetics · 2023Article
- Measuring compound eye optics with microscope and microCT images.Communications biology · 2023Article
- Review
- Article
- Distinctive alteration in the expression of autophagy genes in Drosophila models of amyloidopathy and tauopathy.Upsala journal of medical sciences · 2020Article
- Machine Learning Representation of Loss of Eye Regularity in aFrontiers in neuroscience · 2020Article
- Protecting cells by protecting their vulnerable lysosomes: Identification of a new mechanism for preserving lysosomal functional integrity upon oxidative stress.PLoS genetics · 2017Article
- Quantitative Assessment of Eye Phenotypes for Functional Genetic Studies Using Drosophila melanogaster.G3 (Bethesda, Md.) · 2016Article
- Lazarillo-related Lipocalins confer long-term protection against type I Spinocerebellar Ataxia degeneration contributing to optimize selective autophagy.Molecular neurodegeneration · 2015Article
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5 authors.
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Abstract
The fruitfly compound eye has been broadly used as a model for neurodegenerative diseases. Classical quantitative techniques to estimate the degeneration level of an eye under certain experimental conditions rely either on time consuming histological techniques to measure retinal thickness, or pseudopupil visualization and manual counting. Alternatively, visual examination of the eye surface appearance gives only a qualitative approximation provided the observer is well-trained. Therefore, there is a need for a simplified and standardized analysis of fruitfly eye degeneration extent for both routine laboratory use and for automated high-throughput analysis. We have designed the freely available ImageJ plugin FLEYE, a novel and user-friendly method for quantitative unbiased evaluation of neurodegeneration levels based on the acquisition of fly eye surface pictures. The incorporation of automated image analysis tools and a classification algorithm sustained on a built-in statistical model allow the user to quickly analyze large sample size data with reliability and robustness. Pharmacological screenings or genetic studies using the Drosophila retina as a model system may benefit from our method, because it can be easily implemented in a fully automated environment. In addition, FLEYE can be trained to optimize the image detection capabilities, resulting in a versatile approach to evaluate the pattern regularity of other biological or non-biological samples and their experimental or pathological disruption.
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