ArticleBMC biotechnology2015
Synthesis of Fructooligosaccharides by IslA4, a truncated inulosucrase from Leuconostoc citreum.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 25 citations in OpenAlex.
- Toxicological evaluation of a recombinant inulosucrase (Convero®) produced by precision fermentation:Toxicology reports · 2026Article
- A comprehensive overview of the effects of probiotics, prebiotics and synbiotics on the gut-brain axis.Frontiers in microbiology · 2025Review
- High-yield production and purification of prebiotic inulin-type fructooligosaccharides.AMB Express · 2022Article
- Production of fructan synthesis/hydrolysis of endophytic bacteria involved in inulin production in Jerusalem artichoke.3 Biotech · 2022Article
- Conserved Calcium-Binding Residues at the Ca-I Site Involved in Fructooligosaccharide Synthesis byACS omega · 2020Article
- Extracellular polysaccharides produced by bacteria of the Leuconostoc genus.World journal of microbiology & biotechnology · 2020Review
- Understanding the transfer reaction network behind the non-processive synthesis of low molecular weight levan catalyzed by Bacillus subtilis levansucrase.Scientific reports · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIslA4 is a truncated single domain protein derived from the inulosucrase IslA, which is a multidomain fructosyltransferase produced by Leuconostoc citreum. IslA4 can synthesize high molecular weight inulin from sucrose, with a residual sucrose hydrolytic activity. IslA4 has been reported to retain the product specificity of the multidomain enzyme.
resultsScreening experiments to evaluate the influence of the reactions conditions, especially the sucrose and enzyme concentrations, on IslA4 product specificity revealed that high sucrose concentrations shifted the specificity of the reaction towards fructooligosaccharides (FOS) synthesis, which almost eliminated inulin synthesis and led to a considerable reduction in sucrose hydrolysis. Reactions with low IslA4 activity and a high sucrose activity allowed for high levels of FOS synthesis, where 70% sucrose was used for transfer reactions, with 65% corresponding to transfructosylation for the synthesis of FOS.
conclusionsDomain truncation together with the selection of the appropriate reaction conditions resulted in the synthesis of various FOS, which were produced as the main transferase products of inulosucrase (IslA4). These results therefore demonstrate that bacterial fructosyltransferase could be used for the synthesis of inulin-type FOS.
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