ArticleBMC genomics2015
Sequencing, identification and mapping of primed L1 elements (SIMPLE) reveals significant variation in full length L1 elements between individuals.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Hypomethylating agents increase L1 retroelement expression without inducing novel insertions in myeloid malignancies.Molecular oncology · 2025Article
- Massively parallel jumping assay decodes Alu retrotransposition activity.Nature communications · 2025Article
- MORE-RNAseq: a pipeline for quantifying retrotransposition-capable LINE1 expression based on RNA-seq data.Frontiers in bioinformatics · 2025Article
- Evolutionary insights from profiling LINE-1 activity at allelic resolution in a single human genome.The EMBO journal · 2024Article
- Human Endogenous Retrovirus-K (HML-2)-Related Genetic Variation: Human Genome Diversity and Disease.Genes · 2023Review
- Vitamin C activates young LINE-1 elements in mouse embryonic stem cells via H3K9me3 demethylation.Epigenetics & chromatin · 2023Article
- SARS-CoV-2 and human retroelements: a case for molecular mimicry?BMC genomic data · 2022Article
- Repetitive Elements in Humans.International journal of molecular sciences · 2021Review
- Restriction Enzyme Based Enriched L1Hs Sequencing (REBELseq): A Scalable Technique for Detection of Ta Subfamily L1Hs in the Human Genome.G3 (Bethesda, Md.) · 2020Article
- Human transposon insertion profiling by sequencing (TIPseq) to map LINE-1 insertions in single cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Integrated Mobile Element Scanning (ME-Scan) method for identifying multiple types of polymorphic mobile element insertions.Mobile DNA · 2020Article
- Novel Discovery of LINE-1 in a Korean Individual by a Target Enrichment Method.Molecules and cells · 2019Article
- Computational tools to unmask transposable elements.Nature reviews. Genetics · 2018Review
- A high throughput screen for active human transposable elements.BMC genomics · 2018Article
- Transposable elements in cancer.Nature reviews. Cancer · 2017Review
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- The Human Long Interspersed Element-1 Retrotransposon: An Emerging Biomarker of Neoplasia.Clinical chemistry · 2017Review
- A comprehensive approach to expression of L1 loci.Nucleic acids research · 2017Article
- Human transposon insertion profiling: Analysis, visualization and identification of somatic LINE-1 insertions in ovarian cancer.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transcription coupled repair and biased insertion of human retrotransposon L1 in transcribed genes.Mobile DNA · 2017Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThere are over a half a million copies of L1 retroelements in the human genome which are responsible for as much as 0.5% of new human genetic diseases. Most new L1 inserts arise from young source elements that are polymorphic in the human genome. Highly active polymorphic "hot" L1 source elements have been shown to be capable of extremely high levels of mobilization and result in numerous instances of disease. Additionally, hot polymorphic L1s have been described to be highly active within numerous cancer genomes. These hot L1s result in mutagenesis by insertion of new L1 copies elsewhere in the genome, but also have been shown to generate additional full length L1 insertions which are also hot and able to further retrotranspose. Through this mechanism, hot L1s may amplify within a tumor and result in a continued cycle of mutagenesis. RESULTS AND
conclusionsWe have developed a method to detect full-length, polymorphic L1 elements using a targeted next generation sequencing approach, Sequencing Identification and Mapping of Primed L1 Elements (SIMPLE). SIMPLE has 94% sensitivity and detects nearly all full-length L1 elements in a genome. SIMPLE will allow researchers to identify hot mutagenic full-length L1s as potential drivers of genome instability. Using SIMPLE we find that the typical individual has approximately 100 non-reference, polymorphic L1 elements in their genome. These elements are at relatively low population frequencies relative to previously identified polymorphic L1 elements and demonstrate the tremendous diversity in potentially active L1 elements in the human population.
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Registered trials
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