ArticleBMC research notes2015
Development of Loop-Mediated Isothermal Amplification (LAMP) assay for rapid detection of Fusarium oxysporum f. sp. ciceris - wilt pathogen of chickpea.
Article in BMC research notes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 65 citations in OpenAlex.
- Development of a Field-Deployable Loop-Mediated Isothermal Amplification Assay for the Rapid Detection ofJournal of fungi (Basel, Switzerland) · 2026Article
- From Morphology to Multi-Omics: A New Age of Fusarium Research.Pathogens (Basel, Switzerland) · 2025Review
- Development of rapid and simple detection of bean common mosaic virus (BCMV) in mung beans (Virusdisease · 2025Article
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- Isothermal Amplification Techniques: An Emerging Tool for On-Site Detection of Phytopathogens in Field Conditions.Methods in molecular biology (Clifton, N.J.) · 2025Review
- A comprehensive guide to loop-mediated isothermal amplification, an emerging diagnostic tool for plant pathogenic fungi.Frontiers in plant science · 2025Review
- LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives.Life (Basel, Switzerland) · 2024Review
- Brassicaceae Fungi and Chromista Diseases: Molecular Detection and Host–Plant InteractionPlants (Basel, Switzerland) · 2023Review
- Current and emerging trends in techniques for plant pathogen detection.Frontiers in plant science · 2023Review
- A colorimetric hydroxy naphthol blue based loop-mediated isothermal amplification detection assay targeting the β-tubulin locus ofFrontiers in plant science · 2022Article
- Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria.The American journal of tropical medicine and hygiene · 2020Article
- Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.Frontiers in cellular and infection microbiology · 2020Review
- Article
- Epitypification ofPersoonia · 2019Article
- Establishment of loop-mediated isothermal amplification for rapid detection ofExperimental and therapeutic medicine · 2019Article
- Rapid detection of Puccinia triticina causing leaf rust of wheat by PCR and loop mediated isothermal amplification.PloS one · 2018Article
- Evaluation of a rapid detection for Coxsackievirus B3 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP).Journal of virological methods · 2017Article
- Rapid and sensitive diagnoses of dry root rot pathogen of chickpea (Rhizoctonia bataticola (Taub.) Butler) using loop-mediated isothermal amplification assay.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFusarium oxysporum f. sp. ciceris (Foc), the causal agent of Fusarium wilt is a devastating pathogen of chickpea. In chickpea, various soil borne pathogens produce (s) similar symptoms, therefore cannot be distinguished easily at field level. There is real need for a rapid, inexpensive, and easy to operate and maintain genotyping tool to facilitate accurate disease diagnosis and surveillance for better management of Fusarium wilt outbreaks.
resultsIn this study, we developed a loop-mediated isothermal amplification (LAMP) assay targeting the elongation factor 1 alpha gene sequence for visual detection of Foc. The LAMP reaction was optimal at 63°C for 60 min. When hydroxynaphthol blue (HNB) was added before amplification, samples with Foc DNA developed a characteristic sky blue colour but those without DNA or with the DNA of six other plant pathogenic fungi did not. Results obtained with LAMP and HNB were confirmed when LAMP products were subjected to gel electrophoresis. The detection limit of this LAMP assay for Foc was 10 fg of genomic DNA per reaction, while that of conventional PCR was 100 pg.
conclusionsIn conclusion, it was found that a LAMP assay combined with HNB is simple, rapid, sensitive, and specific. The LAMP assay does not require specialized equipment, hence can be used in the field for the rapid detection of Foc. This is the first report of the use of LAMP assay for the detection of Foc. The presented LAMP method provides a specific, sensitive and rapid diagnostic tool for the distinction of Foc, with the potential to be standardized as a detection method for Foc in endemic areas and will be very useful for monitoring the disease complex in the field further suggesting the management strategies.
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