Evidence map›Paper›PMID 25886597›Full record

ArticlePloS one2015

Transcriptome and genome size analysis of the Venus flytrap.

Michael Krogh Jensen, Josef Korbinian Vogt, Simon Bressendorff, Andaine Seguin-Orlando, Morten Petersen, Thomas Sicheritz-Pontén, John Mundy

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Long-read sequencing uncovers the adaptive topography of a carnivorous plant genome.Proceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  6. Article
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Michael Krogh JensenDepartment of Biology, University of Copenhagen, Copenhagen, Denmark.
Josef Korbinian VogtCenter for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
Simon BressendorffDepartment of Biology, University of Copenhagen, Copenhagen, Denmark.
Andaine Seguin-OrlandoNational High-throughput DNA Sequencing Centre, Copenhagen, Denmark.
Morten PetersenDepartment of Biology, University of Copenhagen, Copenhagen, Denmark.
Thomas Sicheritz-PonténCenter for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
John MundyDepartment of Biology, University of Copenhagen, Copenhagen, Denmark.
University of Copenhagen · DKTechnical University of Denmark · DKHigh Throughput Biology (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The insectivorous Venus flytrap (Dionaea muscipula) is renowned from Darwin's studies of plant carnivory and the origins of species. To provide tools to analyze the evolution and functional genomics of D. muscipula, we sequenced a normalized cDNA library synthesized from mRNA isolated from D. muscipula flowers and traps. Using the Oases transcriptome assembler 79,165,657 quality trimmed reads were assembled into 80,806 cDNA contigs, with an average length of 679 bp and an N50 length of 1,051 bp. A total of 17,047 unique proteins were identified, and assigned to Gene Ontology (GO) and classified into functional categories. A total of 15,547 full-length cDNA sequences were identified, from which open reading frames were detected in 10,941. Comparative GO analyses revealed that D. muscipula is highly represented in molecular functions related to catalytic, antioxidant, and electron carrier activities. Also, using a single copy sequence PCR-based method, we estimated that the genome size of D. muscipula is approx. 3 Gb. Our genome size estimate and transcriptome analyses will contribute to future research on this fascinating, monotypic species and its heterotrophic adaptations.

Indexed as

Genome, PlantTranscriptomeDroseraceaeGene LibraryMolecular Sequence AnnotationRNA, MessengerSequence Analysis, DNARNA, Messenger

Identifiers

PMID25886597
PMCPMC4401711
OpenAlexW2093263562

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.