ArticleBMC microbiology2015
Development of a multiplex-PCR probe system for the proper identification of Klebsiella variicola.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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34 citing papers in PubMed.
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- Novel Virulence Factors DecipheringInternational journal of molecular sciences · 2022Article
- Accurate Identification of Klebsiella variicola by MALDI-TOF Mass Spectrometry in Clinical Microbiology Laboratories.Microbiology spectrum · 2022Article
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- Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- First isolation of Klebsiella variicola from a horse pleural effusion.BMC veterinary research · 2021Article
- Pyelonephritis and Bacteremia Caused byCase reports in infectious diseases · 2021Article
- Genotypic Characterization of ClinicalFrontiers in microbiology · 2021Article
- Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia.PloS one · 2021Article
- Resistance patterns and clinical outcomes of Klebsiella pneumoniae and invasive Klebsiella variicola in trauma patients.PloS one · 2021Observational
- The ZKIR Assay, a Real-Time PCR Method for the Detection ofApplied and environmental microbiology · 2020Article
- Potential ofBiotechnology for biofuels · 2020Article
- Molecular epidemiology of Klebsiella variicola obtained from different sources.Scientific reports · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundKlebsiella variicola was very recently described as a new bacterial species and is very closely related to Klebsiella pneumoniae; in fact, K. variicola isolates were first identified as K. pneumoniae. Therefore, it might be the case that some isolates, which were initially classified as K. pneumoniae, are actually K. variicola. The aim of this study was to devise a multiplex-PCR probe that can differentiate isolates from these sister species.
resultThis work describes the development of a multiplex-PCR method to identify K. variicola. This development was based on sequencing a K. variicola clinical isolate (801) and comparing it to other K. variicola and K. pneumoniae genomes. The phylogenetic analysis showed that K. variicola isolates form a monophyletic group that is well differentiated from K. pneumoniae. Notably, the isolate K. pneumoniae 342 and K. pneumoniae KP5-1 might have been misclassified because in our analysis, both clustered with K. variicola isolates rather than with K. pneumoniae. The multiplex-PCR (M-PCR-1 to 3) probe system could identify K. variicola with high accuracy using the shared unique genes of K. variicola and K. pneumoniae genomes, respectively. M-PCR-1 was used to assay a collection of multidrug-resistant (503) and antimicrobial-sensitive (557) K. pneumoniae clinical isolates. We found K. variicola with a prevalence of 2.1% (23/1,060), of them a 56.5% (13/23) of the isolates were multidrug resistant, and 43.5% (10/23) of the isolates were antimicrobial sensitive. The phylogenetic analysis of rpoB of K. variicola-positive isolates identified by multiplex-PCR support the correct identification and differentiation of K. variicola from K. pneumoniae clinical isolates.
conclusionsThis multiplex-PCR provides the means to reliably identify and genotype K. variicola. This tool could be very helpful for clinical, epidemiological, and population genetics studies of this species. A low but significant prevalence of K. variicola isolates was found, implying that misclassification had occurred previously. We believe that our multiplex-PCR assay could be of paramount importance to understand the population dynamics of K. variicola in both clinical and environmental settings.
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