ArticleBMC infectious diseases2015
Detection and serotyping of pneumococci in community acquired pneumonia patients without culture using blood and urine samples.
Article in BMC infectious diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.
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Who cites it
11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 31 citations in OpenAlex.
- Systematic review on serotypes distribution of pneumococcal pneumonia in adults and the elderly.BMC public health · 2025Pooled it
- Serotype distribution of invasive and non-invasive pneumococcal disease in children ≤5 years of age following the introduction of 10- and 13-valent pneumococcal conjugate vaccines in infant national immunization programs: a systematic literature review.Frontiers in public health · 2025Pooled it
- Non-lytic antibiotic treatment in community-acquired pneumococcal pneumonia does not attenuate inflammation: the PRISTINE trial.The Journal of antimicrobial chemotherapy · 2019Trial
- Molecular Diagnostics, Antimicrobial Resistance Patterns, and Clinical Outcomes in Hospitalized Pneumonia Patients: A Prospective Study from Jordan and a Call for National Guideline Integration.Infection and drug resistance · 2026Article
- Electrochemical Detection for Isothermal Loop-Mediated Amplification of Pneumolysin Gene ofAnalytical chemistry · 2022Article
- The drop in reported invasive pneumococcal disease among adults during the first COVID-19 wave in the Netherlands explained.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2021Article
- Development of an Extended-Specificity Multiplex Immunoassay for Detection of Streptococcus pneumoniae Serotype-Specific Antigen in Urine by Use of Human Monoclonal Antibodies.Clinical and vaccine immunology : CVI · 2017Article
- Application of capsular sequence typing (CST) to serotype non-viable Streptococcus pneumoniae isolates from an old collection.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Molecular surveillance of nasopharyngeal carriage of Streptococcus pneumoniae in children vaccinated with conjugated polysaccharide pneumococcal vaccines.Scientific reports · 2016Article
- Burden of pneumococcal disease in adults aged 65 years and older: an Australian perspective.Pneumonia (Nathan Qld.) · 2016Article
- Methodological criticisms in the evaluation of Pneumococcal Conjugate Vaccine effectiveness.Journal of preventive medicine and hygiene · 2015Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTreatment of community acquired pneumonia (CAP) patients with antibiotics before laboratory-confirmed diagnosis leads to loss of knowledge on the causative bacterial pathogen. Therefore, an increasing number of pneumococcal infections is identified using non-culture based techniques. However, methods for serotyping directly on the clinical specimen remain scarce. Here we present three approaches for detection and serotyping of pneumococci using samples from patients with CAP.
methodsThe first approach is quantitative PCR (qPCR) analysis on blood samples (n = 211) followed by capsular sequence typing (CST) to identify the serotype. The second approach, a urinary antigen assay (n = 223), designated as inhibition multiplex immunoassay (IMIA), is based on Luminex technology targeting 14 serotypes. The third approach is a multiplex immunoassay (MIA) (n = 171) also based on Luminex technology which detects serologic antibody responses against 14 serotypes. The three alternative assays were performed on samples obtained from 309 adult hospitalized CAP patients in 2007-2010 and the results were compared with those obtained from conventional laboratory methods to detect pneumococcal CAP, i.e. blood cultures, sputum cultures and BinaxNOW urinary antigen tests.
resultsUsing qPCR, MIA and IMIA, we were able to detect the pneumococcus in samples of 56% more patients compared to conventional methods. Furthermore, we were able to assign a serotype to the infecting pneumococcus from samples of 25% of all CAP patients, using any of the three serotyping methods (CST, IMIA and MIA).
conclusionThis study indicates the usefulness of additional molecular methods to conventional laboratory methods for the detection of pneumococcal pneumonia. Direct detection and subsequent serotyping on clinical samples will improve the accuracy of pneumococcal surveillance to monitor vaccine effectiveness.
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