ArticleDiagnostic pathology2015
3-dimensional digital reconstruction of the murine coronary system for the evaluation of chronic allograft vasculopathy.
Article in Diagnostic pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Towards Realistic 3D Models of Tumor Vascular Networks.Cancers · 2023Article
- Artificial intelligence and algorithmic computational pathology: an introduction with renal allograft examples.Histopathology · 2021Review
- Banff Digital Pathology Working Group: Going digital in transplant pathology.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2020Article
- An intuitive explanation of dermoscopic structures by digitally reconstructed pathological horizontal top-down view images.Scientific reports · 2019Article
- Introduction to Digital Image Analysis in Whole-slide Imaging: A White Paper from the Digital Pathology Association.Journal of pathology informatics · 2019Review
- Transformation diffusion reconstruction of three-dimensional histology volumes from two-dimensional image stacks.Medical image analysis · 2017Article
- Three-dimensional Imaging and Scanning: Current and Future Applications for Pathology.Journal of pathology informatics · 2017Article
- Optimized SIFTFlow for registration of whole-mount histology to reference optical images.Journal of medical imaging (Bellingham, Wash.) · 2016Article
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Authors and funding
8 authors.
Funding
Abstract
backgroundChronic allograft vasculopathy (CAV) is a major mechanism of graft failure of transplanted organs in humans. Morphometric analysis of coronary arteries enables the quantitation of CAV in mouse models of heart transplantation. However, conventional histological procedures using single 2-dimensional sections limit the accuracy of CAV quantification. The aim of this study is to improve the accuracy of CAV quantification by reconstructing the murine coronary system in 3-dimensions (3D) and using virtual reconstruction and volumetric analysis to precisely assess neointimal thickness.
methodsMouse tissue samples, native heart and transplanted hearts with chronic allograft vasculopathy, were collected and analyzed. Paraffin embedded samples were serially sectioned, stained and digitized using whole slide digital imaging techniques under normal and ultraviolet lighting. Sophisticated software tools were used to generate and manipulate 3D reconstructions of the major coronary arteries and branches.
resultsThe 3D reconstruction provides not only accurate measurements but also exact volumetric data of vascular lesions. This virtual coronary arteriography demonstrates that the vasculopathy lesions in this model are localized to the proximal coronary segments. In addition, virtual rotation and volumetric analysis enabled more precise measurements of CAV than single, randomly oriented histologic sections, and offer an improved readout for this important experimental model.
conclusionsWe believe 3D reconstruction of 2D histological slides will provide new insights into pathological mechanisms in which structural abnormalities play a role in the development of a disease. The techniques we describe are applicable to the analysis of arteries, veins, bronchioles and similar sized structures in a variety of tissue types and disease model systems. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3772457541477230 .
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