Evidence map›Paper›PMID 25884475›Full record

ArticleBMC research notes2015

Gateway-compatible tissue-specific vectors for plant transformation.

Marta Michniewicz, Elizabeth M Frick, Lucia C Strader

Abstract read
In one paragraph

Article in BMC research notes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

  1. Mutational analyses of an instability domain reveal its conserved role in the regulation of class-B ARF levels inProceedings of the National Academy of Sciences of the United States of America · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Marta MichniewiczDepartment of Biology, Washington University, St. Louis, MO, 63130, USA. martampaciorek@gmail.com.
Elizabeth M FrickDepartment of Biology, Washington University, St. Louis, MO, 63130, USA. fricke@wustl.edu.
Lucia C StraderDepartment of Biology, Washington University, St. Louis, MO, 63130, USA. strader@wustl.edu.

Funding

Using Arabidopsis to uncover interactions between phytohormone signaling pathwaysR00GM089987 · NIGMS · WASHINGTON UNIVERSITY · PI STRADER, LUCIA · 2011 to 2013
$728k
NIGMS NIH HHS R00 GM089987NIGMS NIH HHS R00 GM089987-03
6 · The paper itself

Abstract

backgroundUnderstanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.

findingsTo allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.

conclusionsThese vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.

Indexed as

Gene Transfer TechniquesPromoter Regions, GeneticTransformation, GeneticAgrobacterium tumefaciensAlcohol DehydrogenaseArabidopsisArabidopsis ProteinsBacterial ProteinsBase SequenceChlorophyll Binding ProteinsCloning, MolecularEscherichia coliGenes, ReporterGenetic VectorsLuminescent ProteinsMolecular Sequence DataAlcohol DehydrogenaseArabidopsis ProteinsBacterial ProteinsChlorophyll Binding ProteinsEXPANSIN A7 protein, ArabidopsisLBD16 protein, ArabidopsisLuminescent ProteinsSCR protein, ArabidopsisTranscription FactorsUbiquitinyellow fluorescent protein, Bacteria

Identifiers

PMID25884475
PMCPMC4352289

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.