ArticleScientific reports2015
Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
95 citing papers in PubMed.
- Phylogenetic Authentication of Amplicon Sequence Variants in Single-Specimen Metabarcoding of Tropical Insects.Molecular ecology resources · 2026Article
- Forensic Species Identification: A Case Involving Trafficked Fish Maws and Shark Fins.International journal of molecular sciences · 2026Article
- Three new species of genusZooKeys · 2026Article
- Characterization of Gut Microbiota of Honey Bees in Korea.Polish journal of microbiology · 2025Article
- The CODEX Approach: High-Throughput Sequencing of the Cox-1 Barcode Fragment in Neogastropods (Mollusca, Gastropoda).Molecular ecology resources · 2025Article
- Dietary n-3 polyunsaturated fatty acids enhance dry matter intake in transition cows by regulating taste transduction gene expression in the liver associated with rumen microbial alteration.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Development of a Rapid and Cost-Effective Multiplex PCR Assay for the Simultaneous Identification of Three Commercially Important Sea Squirt Species (Foods (Basel, Switzerland) · 2025Article
- Fast-tracking bespoke DNA reference database generation from museum collections for biomonitoring and conservation.Molecular ecology resources · 2025Article
- DNA barcoding, integrative taxonomy, citizen science, and Bush Blitz surveys combine to reveal 34 new species ofZooKeys · 2025Article
- Article
- A New Species ofInsects · 2024Article
- Temporal Dynamics of Fungal Communities in Alkali-Treated Round Bamboo Deterioration under Natural Weathering.Microorganisms · 2024Article
- More Than 200 Years Later:Insects · 2024Article
- Arthropod diversity in the alpine tundra using metabarcoding: Spatial and temporal differences in alpha- and beta-diversity.Ecology and evolution · 2024Article
- The InBIO Barcoding Initiative Database: DNA barcodes of Iberian Bees.Biodiversity data journal · 2024Article
- Scalable, Cost-Effective, and Decentralized DNA Barcoding with Oxford Nanopore Sequencing.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species.Parasite (Paris, France) · 2024Article
- Development of mitochondrial DNA cytochromeBiodiversity data journal · 2024Article
- The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese moths.Biodiversity data journal · 2024Article
- The InBIO Barcoding Initiative Database: DNA barcodes of Orthoptera from Portugal.Biodiversity data journal · 2024Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic information is a valuable component of biosystematics, especially specimen identification through the use of species-specific DNA barcodes. Although many genomics applications have shifted to High-Throughput Sequencing (HTS) or Next-Generation Sequencing (NGS) technologies, sample identification (e.g., via DNA barcoding) is still most often done with Sanger sequencing. Here, we present a scalable double dual-indexing approach using an Illumina Miseq platform to sequence DNA barcode markers. We achieved 97.3% success by using half of an Illumina Miseq flowcell to obtain 658 base pairs of the cytochrome c oxidase I DNA barcode in 1,010 specimens from eleven orders of arthropods. Our approach recovers a greater proportion of DNA barcode sequences from individuals than does conventional Sanger sequencing, while at the same time reducing both per specimen costs and labor time by nearly 80%. In addition, the use of HTS allows the recovery of multiple sequences per specimen, for deeper analysis of genetic variation in target gene regions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.