ArticleScientifica2015
Effects of the Probiotic Enterococcus faecium and Pathogenic Escherichia coli Strains in a Pig and Human Epithelial Intestinal Cell Model.
Article in Scientifica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Investigating the therapeutic effects of caffeine-naringenin combination on the repair of intestinal permeability and composition of gut microbiota in obese rats under long-term high-fat diet.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Bio-Preservation Potential and Antimicrobial Activity of Bacteriocin-Producing Lactic Acid Bacteria Isolated from Ethiopian Traditional Fermented Dairy Products.Probiotics and antimicrobial proteins · 2025Article
- Probiotic potential and antimicrobial efficacy of a dairy isolate, Enterococcus faecium MBBL3.Applied microbiology and biotechnology · 2025Article
- Dynamic Interactions between Diarrhoeagenic EnteroaggregativeMicroorganisms · 2023Article
- Effects ofAnimals : an open access journal from MDPI · 2023Article
- The Effect of Maternal Probiotic or Synbiotic Supplementation on Sow and Offspring Gastrointestinal Microbiota, Health, and Performance.Animals : an open access journal from MDPI · 2023Review
- Effects ofMicroorganisms · 2022Article
- Effect ofNutrients · 2022Article
- Overview of the Importance of Biotics in Gut Barrier Integrity.International journal of molecular sciences · 2022Review
- Norepinephrine and Serotonin Can Modulate the Behavior of the ProbioticMicroorganisms · 2022Article
- Article
- Article
- High-quality genome sequence assembly of R.A73 Enterococcus faecium isolated from freshwater fish mucus.BMC microbiology · 2020Article
- Potential Probiotic Lactobacillus rhamnosus (MTCC-5897) Inhibits Escherichia coli Impaired Intestinal Barrier Function by Modulating the Host Tight Junction Gene Response.Probiotics and antimicrobial proteins · 2020Article
- Inflammatory Responses of Porcine MoDC and Intestinal Epithelial Cells in a Direct-Contact Co-culture System Following a Bacterial Challenge.Inflammation · 2020Article
- Probiotic Potential and Safety Evaluation ofFrontiers in microbiology · 2019Article
- The Inflammatory Response to EnterotoxigenicMediators of inflammation · 2018Article
- Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli.International journal of molecular sciences · 2017Article
- EnterotoxigenicBioscience of microbiota, food and health · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study has been to elucidate the effect of the probiotic Enterococcus faecium NCIMB 10415 on epithelial integrity in intestinal epithelial cells and whether pre- and coincubation with this strain can reproducibly prevent damage induced by enterotoxigenic (ETEC) and enteropathogenic Escherichia coli (EPEC). Porcine (IPEC-J2) and human (Caco-2) intestinal epithelial cells were incubated with bacterial strains and epithelial integrity was assessed by measuring transepithelial electrical resistance (TEER) and mannitol flux rates. E. faecium alone increased TEER of Caco-2 cells without affecting mannitol fluxes whereas the E. coli strains decreased TEER and concomitantly increased mannitol flux rates in both cell lines. Preincubation with E. faecium had no effect on the TEER decrease induced by E. coli in preliminary experiments. However, in a second set of experiments using a slightly different protocol, E. faecium ameliorated the TEER decrease induced by ETEC at 4 h in IPEC-J2 and at 2, 4, and 6 h in Caco-2 cells. We conclude that E. faecium positively affected epithelial integrity in monoinfected Caco-2 cells and could ameliorate the damage on TEER induced by an ETEC strain. Reproducibility of the results is, however, limited when experiments are performed with living bacteria over longer periods.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.