ArticleEuropean journal of microbiology & immunology2015
Comparison of an automated nucleic acid extraction system with the column-based procedure.
Article in European journal of microbiology & immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
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- Interaction of Nanomaterials with Nucleic Acids and Their Applications in Nucleic Acid Analysis.International journal of biological sciences · 2025Review
- Automated total nucleic acid extraction with magnetic beads for the detection of Plasmodium falciparum in large study cohorts.Malaria journal · 2024Article
- Research on a Magnetic Separation-Based Rapid Nucleic Acid Extraction System and Its Detection Applications.Biosensors · 2023Article
- Metagenomic Sequencing for the Diagnosis ofInternational journal of molecular sciences · 2022Article
- Multicentric Evaluation of SeeGene Allplex Real-Time PCR Assays Targeting 28 Bacterial, Microsporidal and Parasitic Nucleic Acid Sequences in Human Stool Samples.Diagnostics (Basel, Switzerland) · 2022Article
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- Comparison of Two Real-Time PCR Assays Targeting Ribosomal Sequences for the Identification ofPathogens (Basel, Switzerland) · 2021Article
- Comparison of Three In-House Real PCR Assays Targeting Kinetoplast DNA, the Small Subunit Ribosomal RNA Gene and the Glucose-6-Phosphate Isomerase Gene for the Detection ofPathogens (Basel, Switzerland) · 2021Article
- New Developments in PCR-Based Diagnostics for Bacterial Pathogens Causing Gastrointestinal Infections-A Narrative Mini-Review on Challenges in the Tropics.Tropical medicine and infectious disease · 2021Review
- Detection of 12 Common Food-Borne Bacterial Pathogens by TaqMan Real-Time PCR Using a Single Set of Reaction Conditions.Frontiers in microbiology · 2019Article
- Poor Diagnostic Performance of a Species-Specific Loop-Mediated Isothermal Amplification (LAMP) Platform for Malaria.European journal of microbiology & immunology · 2018Article
- Closed-type pre-treatment device for point-of-care testing of sputum.Scientific reports · 2018Article
- Microbiological screenings for infection control in unaccompanied minor refugees: the German Armed Forces Medical Service's experience.Military Medical Research · 2017Article
- More Pathogenicity or Just More Pathogens?-On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays.Frontiers in microbiology · 2017Review
- Comparison of two real-time PCR assays for the detection of malaria parasites from hemolytic blood samples - Short communication.European journal of microbiology & immunology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we assessed the extraction efficiency of a deployable bench-top nucleic acid extractor EZ1 in comparison to the column-based approach with complex sample matrices. A total of 48 EDTA blood samples and 81 stool samples were extracted by EZ1 automated extraction and the column-based QIAamp DNA Mini Kit. Blood sample extractions were assessed by two real-time malaria PCRs, while stool samples were analyzed by six multiplex real-time PCR assays targeting bacterial, viral, and parasitic stool pathogens. Inhibition control PCR testing was performed as well. In total, 147 concordant and 13 discordant pathogen-specific PCR results were obtained. The latter comprised 11 positive results after column-based extraction only and two positive results after EZ1 extraction only. EZ1 extraction showed a higher frequency of inhibition. This phenomenon was, however, inconsistent for the different PCR schemes. In case of concordant PCR results, relevant differences of cycle threshold numbers for the compared extraction schemes were not observed. Switches from well-established column-based extraction to extraction with the automated EZ1 system do not lead to a relevantly reduced yield of target DNA when complex sample matrices are used. If sample inhibition is observed, column-based extraction from another sample aliquot may be considered.
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