ArticleNucleic acids research2015
Thioflavin T as a fluorescence probe for monitoring RNA metabolism at molecular and cellular levels.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 86 citations in OpenAlex.
- The role of the 2'-OH group in phase separation and percolation transitions of RNA.Nature communications · 2026Article
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- The Critical Role of the 2'-OH group in Phase Separation and Percolation Transitions of RNA.bioRxiv : the preprint server for biology · 2026Article
- Uncovering a previously unknown function of polyphosphate in polyadenylated RNA-induced amyloidogenesis of Hfq.The FEBS journal · 2026Article
- Molecular and Structural Characterization Reveals Divergent Extracellular Vesicle Profiles Between Wild Type and Alzheimer's Disease Cerebrocortical Organoids.bioRxiv : the preprint server for biology · 2026Article
- BR-bodies facilitate adaptive responses and survival during copper stress in Caulobacter crescentus.The Journal of biological chemistry · 2025Article
- Effect of Pefloxacin onAntibiotics (Basel, Switzerland) · 2025Article
- RNA G‑Quadruplex Reprogramming with Guanine-Rich Antisense Oligonucleotides Inhibits Monoamine Oxidase B's Translation.ACS bio & med chem Au · 2025Article
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- Tailoring and reversing m6A editing with sequential RNA bioorthogonal chemistry.Nucleic acids research · 2025Article
- BR-Bodies Facilitate Adaptive Responses and Survival During Copper Stress inbioRxiv : the preprint server for biology · 2025Article
- Thioflavin-T: application as a neuronal body and nucleolar stain and the blue light photo enhancement effect.Scientific reports · 2024Article
- Electrical Capacitance Tomography of Cell Cultures on a CMOS Microelectrode Array.IEEE transactions on biomedical circuits and systems · 2024Article
- Effect of Clemizole on Alpha-Synuclein-Preformed Fibrils-Induced Parkinson's Disease Pathology: A Pharmacological Investigation.Neuromolecular medicine · 2024Article
- Topological effect of an intramolecular split G-quadruplex on thioflavin T binding and fluorescence light-up.Chemical science · 2024Article
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- Ferroptosis induces nucleolar stress as revealed by live-cell imaging using thioflavin T.Current research in pharmacology and drug discovery · 2024Article
- Recent Advances in Fluorescent Probes for G-quadruplex DNAs / RNAs.Mini reviews in medicinal chemistry · 2024Review
- Thioflavin T indicates mitochondrial membrane potential in mammalian cells.Biophysical reports · 2023Article
- RNA sequestration driven by amyloid formation: the alpha synuclein case.Nucleic acids research · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intrinsically stochastic dynamics of mRNA metabolism have important consequences on gene regulation and non-genetic cell-to-cell variability; however, no generally applicable methods exist for studying such stochastic processes quantitatively. Here, we describe the use of the amyloid-binding probe Thioflavin T (ThT) for monitoring RNA metabolism in vitro and in vivo. ThT fluoresced strongly in complex with bacterial total RNA than with genomic DNA. ThT bound purine oligoribonucleotides preferentially over pyrimidine oligoribonucleotides and oligodeoxyribonucleotides. This property enabled quantitative real-time monitoring of poly(A) synthesis and phosphorolysis by polyribonucleotide phosphorylase in vitro. Cellular analyses, in combination with genetic approaches and the transcription-inhibitor rifampicin treatment, demonstrated that ThT mainly stained mRNA in actively dividing Escherichia coli cells. ThT also facilitated mRNA metabolism profiling at the single-cell level in diverse bacteria. Furthermore, ThT can also be used to visualise transitions between non-persister and persister cell states, a phenomenon of isogenic subpopulations of antibiotic-sensitive bacteria that acquire tolerance to multiple antibiotics due to stochastically induced dormant states. Collectively, these results suggest that probing mRNA dynamics with ThT is a broadly applicable approach ranging from the molecular level to the single-cell level.
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