ArticleLab on a chip2015
Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it, 141 citations in OpenAlex.
- Applications of Lab on a Chip in Antimicrobial Susceptibility ofMedicina (Kaunas, Lithuania) · 2023Pooled it
- Artificial Intelligence-Empowered Single-Cell Phenotyping for Rapid, Automated Pathogen Diagnostics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Recent advances in the biosensing platforms for sepsis diagnosis.RSC advances · 2026Review
- A novel width-expanding spiral microchannel for high-throughput continuous separation of bacterial-sized particles.Scientific reports · 2026Article
- Dean migration mechanisms: facilitating the control of microbial contaminants in cell therapy product manufacturing.Microsystems & nanoengineering · 2026Article
- Culture-free detection of bacteria from blood for rapid sepsis diagnosis.NPJ digital medicine · 2025Article
- Isolation and identification of bacteria from blood within 12 h using standard laboratory equipment.Scientific reports · 2025Article
- Advances in Host Depletion and Pathogen Enrichment Methods for Rapid Sequencing-Based Diagnosis of Bloodstream Infection.The Journal of molecular diagnostics : JMD · 2024Review
- Simultaneous high-throughput particle-bacteria separation and solution exchange via in-plane and out-of-plane parallelization of microfluidic centrifuges.Biomicrofluidics · 2024Article
- Separation of Activated T Cells Using Multidimensional Double Spiral (MDDS) Inertial Microfluidics for High-Efficiency CAR T Cell Manufacturing.Analytical chemistry · 2024Article
- Microfluidic technologies for advanced antimicrobial susceptibility testing.Biomicrofluidics · 2024Review
- Automated sepsis detection with vancomycin- and allantoin-polydopamine magnetic nanoparticles.Scientific reports · 2024Article
- Numerical investigation of heterogeneous soft particle pairs in inertial microfluidics.Soft matter · 2024Article
- Enhanced functionalities of immune cells separated by a microfluidic lattice: assessment based on holotomography.Biomedical optics express · 2023Article
- Development of an Optically Induced Dielectrophoresis (ODEP) Microfluidic System for High-Performance Isolation and Purification of Bacteria.Biosensors · 2023Article
- A Systematic Review of In Vitro Studies Using Microchip Platforms for Identifying Periodontopathogens from the Red Complex.Dentistry journal · 2023Review
- Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review.Sensors (Basel, Switzerland) · 2023Review
- Current and Future Flow Cytometry Applications Contributing to Antimicrobial Resistance Control.Microorganisms · 2023Review
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- A novel rRNA hybridization-based approach to rapid, accurate Candida identification directly from blood culture.Medical mycology · 2022Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Detection of bacteria in bloodstream infections and their antibiotic susceptibility patterns is critical to guide therapeutic decision-making for optimal patient care. Current culture-based assays are too slow (>48 h), leading to excessive up-front use of broad-spectrum antibiotics and/or incorrect antibiotic choices due to resistant bacteria, each with deleterious consequences for patient care and public health. To approach this problem, we describe a method to rapidly isolate bacteria from whole blood using inertial microfluidics and directly determine pathogen identity and antibiotic susceptibility with hybridization-based RNA detection. Using the principle of Dean flow fractionation, bacteria are separated from host blood cells in a label-free separation method with efficient recovery of even low abundance bacteria. Ribosomal RNA detection can then be applied for direct identification of low abundance pathogens (~100 per mL) from blood without culturing or enzymatic amplification. Messenger RNA detection of antibiotic-responsive transcripts after brief drug exposure permits rapid susceptibility determination from bacteria with minimal culturing (~10(5) per mL). This unique coupling of microfluidic cell separation with RNA-based molecular detection techniques represents significant progress towards faster diagnostics (~8 hours) to guide antibiotic therapy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.