ArticleNature protocols2015
High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
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- PepSeq as a highly multiplexed platform for melioidosis antigen discovery and vaccine development.Frontiers in immunology · 2025Article
- Monitoring host-pathogen interactions using chemical proteomics.RSC chemical biology · 2024Review
- AMPylation profiling during neuronal differentiation reveals extensive variation on lysosomal proteins.iScience · 2021Article
- MS-BasedACS infectious diseases · 2020Article
- COVID-19 diagnostic testing: Technology perspective.Clinical and translational medicine · 2020Article
- In-depth serum proteomics reveals biomarkers of psoriasis severity and response to traditional Chinese medicine.Theranostics · 2019Article
- Self-assembling functional programmable protein array for studying protein-protein interactions in malaria parasites.Malaria journal · 2018Article
- cat-ELCCA: catalyzing drug discovery through click chemistry.Chemical communications (Cambridge, England) · 2018Article
- Legionella effector AnkX interacts with host nuclear protein PLEKHN1.BMC microbiology · 2018Article
- Quantitative proteomics in lung cancer.Journal of biomedical science · 2017Review
- Discovering Protein-Protein Interactions Using Nucleic Acid Programmable Protein Arrays.Current protocols in cell biology · 2017Article
- Multiplexed Nucleic Acid Programmable Protein Arrays.Theranostics · 2017Article
- A Microwell-Printing Fabrication Strategy for the On-Chip Templated Biosynthesis of Protein Microarrays for Surface Plasmon Resonance Imaging.The journal of physical chemistry. C, Nanomaterials and interfaces · 2016Article
- A click chemistry-based microRNA maturation assay optimized for high-throughput screening.Chemical communications (Cambridge, England) · 2016Article
- Ultrasensitive interferometric on-chip microscopy of transparent objects.Science advances · 2016Article
- Review
- The ORFeome Collaboration: a genome-scale human ORF-clone resource.Nature methods · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
AMPylation (adenylylation) has been recognized as an important post-translational modification that is used by pathogens to regulate host cellular proteins and their associated signaling pathways. AMPylation has potential functions in various cellular processes, and it is widely conserved across both prokaryotes and eukaryotes. However, despite the identification of many AMPylators, relatively few candidate substrates of AMPylation are known. This is changing with the recent development of a robust and reliable method for identifying new substrates using protein microarrays, which can markedly expand the list of potential substrates. Here we describe procedures for detecting AMPylated and auto-AMPylated proteins in a sensitive, high-throughput and nonradioactive manner. The approach uses high-density protein microarrays fabricated using nucleic acid programmable protein array (NAPPA) technology, which enables the highly successful display of fresh recombinant human proteins in situ. The modification of target proteins is determined via copper-catalyzed azide-alkyne cycloaddition (CuAAC). The assay can be accomplished within 11 h.
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