ArticleBMC developmental biology2015
DNA methylation dynamics at imprinted genes during bovine pre-implantation embryo development.
Article in BMC developmental biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- DNA Methylation in the Ovary and Uterus of Mammalian Animal Models: Implications for Reproductive Function.Genes · 2026Review
- Dynamic methylation pattern of H19DMR and KvDMR1 in bovine oocytes and preimplantation embryos.Journal of assisted reproduction and genetics · 2024Article
- Pleomorphic adenoma gene1 in reproduction and implication for embryonic survival in cattle: a review.Journal of animal science · 2024Review
- Update on the Epigenomic Implication of Embryo Cryopreservation Methods Applied in Assisted Reproductive Technologies With Potential Long-Term Health Effects.Frontiers in cell and developmental biology · 2022Review
- Establishment of bovine expanded potential stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Epigenetics of Gametes and Early Embryos and Potential Long-Range Consequences in Livestock Species-Filling in the Picture With Epigenomic Analyses.Frontiers in genetics · 2021Review
- In Vitro Maturation with Leukemia Inhibitory Factor Prior to the Vitrification of Bovine Oocytes Improves Their Embryo Developmental Potential and Gene Expression in Oocytes and Embryos.International journal of molecular sciences · 2020Article
- DNA methylation changes during preimplantation development reveal inter-species differences and reprogramming events at imprinted genes.Clinical epigenetics · 2020Article
- The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis.Scientific reports · 2019Article
- Methylome Dynamics of Bovine Gametes andFrontiers in genetics · 2019Article
- DNA methylomes of bovine gametes and in vivo produced preimplantation embryos.Biology of reproduction · 2018Article
- Article
- Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element.Cellular & molecular biology letters · 2018Article
- Article
- The expression of DNA methyltransferases3A is specifically downregulated in chorionic villi of early embryo growth arrest cases.Molecular medicine reports · 2017Article
- PEG10 overexpression induced by E2F-1 promotes cell proliferation, migration, and invasion in pancreatic cancer.Journal of experimental & clinical cancer research : CR · 2017Article
- Methylation Status of H19/IGF2 Differentially Methylated Region in in vitro Human Blastocysts Donated by Healthy CouplesIranian biomedical journal · 2017Article
- Article
- Epigenetics: A key paradigm in reproductive health.Clinical and experimental reproductive medicine · 2016Review
- mRNA Levels of Imprinted Genes in Bovine In Vivo Oocytes, Embryos and Cross Species Comparisons with Humans, Mice and Pigs.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn mammals, maternal differentially methylated regions (DMRs) acquire DNA methylation during the postnatal growth stage of oogenesis, with paternal DMRs acquiring DNA methylation in the perinatal prospermatagonia. Following fusion of the male and female gametes, it is widely accepted that murine DNA methylation marks at the DMRs of imprinted genes are stable through embryogenesis and early development, until they are reprogrammed in primordial germ cells. However, the DNA methylation dynamics at DMRs of bovine imprinted genes during early stages of development remains largely unknown. The objective of this investigation was to analyse the methylation dynamics at imprinted gene DMRs during bovine embryo development, from blastocyst stage until implantation.
resultsTo this end, pyrosequencing technology was used to quantify DNA methylation at DMR-associated CpG dinucleotides of six imprinted bovine genes (SNRPN, MEST, IGF2R, PLAGL1, PEG10 and H19) using bisulfite-modified genomic DNA isolated from individual blastocysts (Day 7); ovoid embryos (Day 14); filamentous embryos (Day 17) and implanting conceptuses (Day 25). For all genes, the degree of DNA methylation was most variable in Day 7 blastocysts compared to later developmental stages (P < 0.05). Furthermore, mining of RNA-seq transcriptomic data and western blot analysis revealed a specific window of expression of DNA methylation machinery genes (including DNMT3A, DNMT3B, TRIM28/KAP1 and DNMT1) and proteins (DNMT3A, DNMT3A2 and DNMT3B) by bovine embryos coincident with imprint stabilization.
conclusionThe findings of this study suggest that the DNA methylation status of bovine DMRs might be variable during the early stages of embryonic development, possibly requiring an active period of imprint stabilization.
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