ArticleBMC genomics2015
Molecular cloning and expression analysis of WRKY transcription factor genes in Salvia miltiorrhiza.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 115 citations in OpenAlex.
- The Jasmonate-Responsive SmMPK3-SmWRKY33 Module Positively Regulates Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2026Article
- Transcriptome analysis and genome-wide identification of WRKY gene family in Leonurus japonicus under drought stress.BMC plant biology · 2025Article
- Genome-wide identification and characterization of WRKYs family involved in responses to Cylindrocarpon destructans in Panax notoginseng.BMC genomics · 2025Article
- Comparative analysis of the WRKY gene family between Chimonanthus praecox and C. salicifolius.Genetica · 2025Article
- Genome-wide comprehensive analysis the molecular phylogenetic evolution, functional divergence and tissue-specific expression ofFrontiers in plant science · 2025Article
- Genome-wide identification and expression analysis of the WRKY transcription factors related to sesquiterpenes biosynthesis inFrontiers in genetics · 2025Article
- CsWRKY29, a key transcription factor in tea plant for freezing tolerance, ABA sensitivity, and sugar metabolism.Scientific reports · 2024Article
- Genome-Wide Identification and Expression Analysis of Sucrose Nonfermenting 1-Related Protein Kinase (Plants (Basel, Switzerland) · 2024Article
- Genome-wide profiling of WRKY genes involved in flavonoid biosynthesis inFrontiers in plant science · 2024Article
- Phenolic Acids and Flavonoids Play Important Roles in Flower Bud Differentiation inInternational journal of molecular sciences · 2023Article
- Article
- Overexpression of a WRKY transcription factor McWRKY57-like from Mentha canadensis L. enhances drought tolerance in transgenic Arabidopsis.BMC plant biology · 2023Article
- Jasmonic acid regulates the biosynthesis of medicinal metabolites via the JAZ9-MYB76 complex inHorticulture research · 2023Article
- Article
- Genome-Wide Identification and Characterization of the WRKY Gene Family inInternational journal of molecular sciences · 2022Article
- WRKY transcription factors and plant defense responses: latest discoveries and future prospects.Plant cell reports · 2021Review
- Transcription Factor: A Powerful Tool to Regulate Biosynthesis of Active Ingredients inFrontiers in plant science · 2021Review
- Comparative transcriptome analyses of differentPeerJ · 2021Article
- Identification and Application of BhAPRR2 Controlling Peel Colour in Wax Gourd (Frontiers in plant science · 2021Article
- Functional analysis ofPeerJ · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWRKY proteins comprise a large family of transcription factors and play important regulatory roles in plant development and defense response. The WRKY gene family in Salvia miltiorrhiza has not been characterized.
resultsA total of 61 SmWRKYs were cloned from S. miltiorrhiza. Multiple sequence alignment showed that SmWRKYs could be classified into 3 groups and 8 subgroups. Sequence features, the WRKY domain and other motifs of SmWRKYs are largely conserved with Arabidopsis AtWRKYs. Each group of WRKY domains contains characteristic conserved sequences, and group-specific motifs might attribute to functional divergence of WRKYs. A total of 17 pairs of orthologous SmWRKY and AtWRKY genes and 21 pairs of paralogous SmWRKY genes were identified. Maximum likelihood analysis showed that SmWRKYs had undergone strong selective pressure for adaptive evolution. Functional divergence analysis suggested that the SmWRKY subgroup genes and many paralogous SmWRKY gene pairs were divergent in functions. Various critical amino acids contributed to functional divergence among subgroups were detected. Of the 61 SmWRKYs, 22, 13, 4 and 1 were predominantly expressed in roots, stems, leaves, and flowers, respectively. The other 21 were mainly expressed in at least two tissues analyzed. In S. miltiorrhiza roots treated with MeJA, significant changes of gene expression were observed for 49 SmWRKYs, of which 26 were up-regulated, 18 were down-regulated, while the other 5 were either up-regulated or down-regulated at different time-points of treatment. Analysis of published RNA-seq data showed that 42 of the 61 identified SmWRKYs were yeast extract and Ag(+)-responsive. Through a systematic analysis, SmWRKYs potentially involved in tanshinone biosynthesis were predicted.
conclusionThese results provide insights into functional conservation and diversification of SmWRKYs and are useful information for further elucidating SmWRKY functions.
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