ArticleBMC cell biology2015
Ex vivo analysis of renal proximal tubular cells.
Article in BMC cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Isolation and functional characterization of primary human proximal tubular epithelial cells from brain-dead organ donors.Physiological reports · 2026Article
- DNA damage in proximal tubules triggers systemic metabolic dysfunction through epigenetically altered macrophages.Nature communications · 2025Article
- hTERT and SV40LgT Renal Cell Lines Adjust Their Transcriptional Responses After Copy Number Changes from the Parent Proximal Tubule Cells.International journal of molecular sciences · 2025Article
- Glutamine prevents acute kidney injury by modulating oxidative stress and apoptosis in tubular epithelial cells.JCI insight · 2022Article
- Remote ischemic preconditioning causes transient cell cycle arrest and renal protection by a NF-κB-dependent Sema5B pathway.JCI insight · 2022Article
- Transfusion-Related Renal Dysfunction After Cardiac Surgery: The Role of Myeloid-Related Protein_14 in Neutrophil-Mediated Tubular Damage.JACC. Basic to translational science · 2022Article
- Reducing lipid bilayer stress by monounsaturated fatty acids protects renal proximal tubules in diabetes.eLife · 2022Article
- DNA repair factor KAT5 prevents ischemic acute kidney injury through glomerular filtration regulation.iScience · 2021Article
- Renal proximal tubular epithelial cells: review of isolation, characterization, and culturing techniques.Molecular biology reports · 2020Review
- Altered proximal tubular cell glucose metabolism during acute kidney injury is associated with mortality.Nature metabolism · 2020Article
- Rapamycin and dexamethasone during pregnancy prevent tuberous sclerosis complex-associated cystic kidney disease.JCI insight · 2020Article
- The cannabinoid receptor 1 is involved in renal fibrosis during chronic allograft dysfunction: Proof of concept.Journal of cellular and molecular medicine · 2019Article
- Heterogeneous Nuclear Ribonucleoprotein F Mediates Insulin Inhibition of Bcl2-Modifying Factor Expression and Tubulopathy in Diabetic Kidney.Scientific reports · 2019Article
- Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function.Scientific reports · 2019Article
- Cell-specific qRT-PCR of renal epithelial cells reveals a novel innate immune signature in murine collecting duct.American journal of physiology. Renal physiology · 2018Article
- Stress Response Gene Nupr1 Alleviates Cyclosporin A Nephrotoxicity In Vivo.Journal of the American Society of Nephrology : JASN · 2017Article
- Direct reprogramming of fibroblasts into renal tubular epithelial cells by defined transcription factors.Nature cell biology · 2016Article
- Epigenetic changes during sepsis: on your marks!Critical care (London, England) · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExperimental models are inevitably a compromise between accurately reproducing a pathological situation and schematically simplifying it, which is intended to provide both relevance and conclusiveness. In-vivo models are very relevant, but multiple cell-types undergoing various changes may hinder the observation of individual molecular events.
resultsHere, we describe a method for analyzing and isolating specific cell types from the kidney and studying the phenotype they have acquired in vivo. Using flow cytometry, immunofluorescence, and RT-PCR, we show that our method is suitable for studying and isolating proximal tubular cells with an anti Prominin-1 antibody. Kidneys are subjected to mechanical dissociation followed by flow-cytometry analysis. Hundreds of thousands of proximal tubular cells are then isolated by magnetic separation followed by direct analysis or primary cell culture. Using our method, we detect phenotypic changes in the proximal tubular cells after renal ischemia reperfusion, and we isolate the proximal tubular cells, with a purity over 80%.
conclusionsThis method is efficient, quick, simple, and cheap, and should be useful for studying cell-type specific parameters after in vivo experimental studies. It is also a simple method to obtain a specific primary cell culture from any animal strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.