ArticleMicrobial cell factories2015
Fungal cytochrome P450 monooxygenases of Fusarium oxysporum for the synthesis of ω-hydroxy fatty acids in engineered Saccharomyces cerevisiae.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Nanoplastics and fungi: exploring dual roles in degradation and pathogenicity.Frontiers in microbiology · 2025Review
- Three strategy rules of filamentous fungi in hydrocarbon remediation: an overview.Biodegradation · 2024Review
- Glycosylation of Quercetin by Selected Entomopathogenic Filamentous Fungi and Prediction of Its Products' Bioactivity.International journal of molecular sciences · 2023Article
- Role of Omega-Hydroxy Ceramides in Epidermis: Biosynthesis, Barrier Integrity and Analyzing Method.International journal of molecular sciences · 2023Review
- Filamentous fungi for sustainable remediation of pharmaceutical compounds, heavy metal and oil hydrocarbons.Frontiers in bioengineering and biotechnology · 2023Review
- Biodegradation of Petroleum Hydrocarbons byMolecules (Basel, Switzerland) · 2022Article
- Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories.Engineering microbiology · 2022Review
- Hemoprotein Catalyzed Oxygenations: P450s, UPOs, and Progress toward Scalable Reactions.JACS Au · 2021Review
- Bacteria, Fungi and Microalgae for the Bioremediation of Marine Sediments Contaminated by Petroleum Hydrocarbons in the Omics Era.Microorganisms · 2021Review
- Application of Random Mutagenesis and Synthetic FadR Promoter forFrontiers in bioengineering and biotechnology · 2021Article
- Article
- Heterologous coexpression of the benzoate-para-hydroxylase CYP53B1 with different cytochrome P450 reductases in various yeasts.Microbial biotechnology · 2019Article
- Cascade biotransformation of dehydroepiandrosterone (DHEA) by Beauveria species.Scientific reports · 2018Article
- Identification of the decumbenone biosynthetic gene cluster inFungal biology and biotechnology · 2018Article
- Condition-dependent co-regulation of genomic clusters of virulence factors in the grapevine trunk pathogen Neofusicoccum parvum.Molecular plant pathology · 2018Article
- Microbial synthesis of medium-chain chemicals from renewables.Nature biotechnology · 2017Review
- Succinct synthesis of saturated hydroxy fatty acids andMedChemComm · 2017Article
- Diverse Metabolic Capacities of Fungi for Bioremediation.Indian journal of microbiology · 2016Review
- Versatile biocatalysis of fungal cytochrome P450 monooxygenases.Microbial cell factories · 2016Review
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8 authors.
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Abstract
backgroundOmega hydroxy fatty acids (ω-OHFAs) are multifunctional compounds that act as the basis for the production of various industrial products with broad commercial and pharmaceutical implications. However, the terminal oxygenation of saturated or unsaturated fatty acids for the synthesis of ω-OHFAs is intricate to accomplish through chemocatalysis, due to the selectivity and controlled reactivity in C-H oxygenation reactions. Cytochrome P450, the ubiquitous enzyme is capable of catalyzing the selective terminal omega hydroxylation naturally in biological kingdom.
resultsTo gain a deep insight on the biochemical role of fungal P450s towards the production of omega hydroxy fatty acids, two cytochrome P450 monooxygenases from Fusarium oxysporum (FoCYP), FoCYP539A7 and FoCYP655C2; were identified, cloned, and heterologously expressed in Saccharomyces cerevisiae. For the efficient production of ω-OHFAs, the S. cerevisiae was engineered to disrupt the acyl-CoA oxidase enzyme and the β-oxidation pathway inactivated (ΔPox1) S. cerevisiae mutant was generated. To elucidate the significance of the interaction of redox mechanism, FoCYPs were reconstituted with the heterologous and homologous reductase systems--S. cerevisiae CPR (ScCPR) and F. oxysporum CPR (FoCPR). To further improve the yield, the effect of pH was analyzed and the homologous FoCYP-FoCPR system efficiently hydroxylated caprylic acid, capric acid and lauric acid into their respective ω-hydroxy fatty acids with 56%, 79% and 67% conversion. Furthermore, based on computational simulations, we identified the key residues (Asn106 of FoCYP539A7 and Arg235 of FoCYP655C2) responsible for the recognition of fatty acids and demonstrated the structural insights of the active site of FoCYPs.
conclusionFungal CYP monooxygenases, FoCYP539A7 and FoCYP655C2 with its homologous redox partner, FoCPR constitutes a promising catalyst due to its high regio- and stereo-selectivity in the hydroxylation of fatty acids and in the substantial production of industrially valuable ω-hydroxy fatty acids.
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