ArticleBMC research notes2015
Characterisation and expression analysis of cathepsins and ubiquitin-proteasome genes in gilthead sea bream (Sparus aurata) skeletal muscle.
Article in BMC research notes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Characterization of myogenesis in European sea bass (Dicentrarchus labrax) using primary white muscle cell cultures.Scientific reports · 2025Article
- Interaction of host gene-gut microbiota in male grading ofMicrobiology spectrum · 2025Article
- The Pattern of Gene Expression (Animals : an open access journal from MDPI · 2024Article
- Role of Enzymatic Reactions in Meat Processing and Use of Emerging Technologies for Process Intensification.Foods (Basel, Switzerland) · 2023Review
- Muscle regeneration in gilthead sea bream: Implications of endocrine and local regulatory factors and the crosstalk with bone.Frontiers in endocrinology · 2023Article
- Gilthead Seabream Liver Integrative Proteomics and Metabolomics Analysis Reveals Regulation by Different Prosurvival Pathways in the Metabolic Adaptation to Stress.International journal of molecular sciences · 2022Article
- Myomaker and Myomixer Characterization in Gilthead Sea Bream under Different Myogenesis Conditions.International journal of molecular sciences · 2022Article
- Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Animals : an open access journal from MDPI · 2021Article
- Diet and Exercise Modulate GH-IGFs Axis, Proteolytic Markers and Myogenic Regulatory Factors in Juveniles of Gilthead Sea Bream (Animals : an open access journal from MDPI · 2021Article
- Regulatory mechanisms involved in muscle and bone remodeling during refeeding in gilthead sea bream.Scientific reports · 2020Article
- Ghrelin and Its Receptors in Gilthead Sea Bream: Nutritional Regulation.Frontiers in endocrinology · 2018Article
- Article
- BAC-pool sequencing and analysis confirms growth-associated QTLs in the Asian seabass genome.Scientific reports · 2016Article
- Adipogenic Gene Expression in Gilthead Sea Bream Mesenchymal Stem Cells from Different Origin.Frontiers in endocrinology · 2016Article
- Genome-Wide Association Study for Identifying Loci that Affect Fillet Yield, Carcass, and Body Weight Traits in Rainbow Trout (Frontiers in genetics · 2016Article
- Uncommon functional properties of the first piscine 26S proteasome from the Antarctic notothenioid Trematomus bernacchii.Bioscience reports · 2016Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe proteolytic enzymes involved in normal protein turnover in fish muscle are also responsible for post-mortem softening of the flesh and are therefore potential determinants of product quality. The main enzyme systems involved are calpains, cathepsins, and the ubiquitin-proteasome (UbP). In this study on Sparus aurata (Sa), the coding sequences of cathepsins (SaCTSB and SaCTSDb) and UbP family members (SaN3 and SaUb) were cloned from fast skeletal muscle, and their expression patterns were examined during ontogeny and in a fasting/re-feeding experiment.
resultsThe amino acid sequences identified shared 66-100% overall identity with their orthologues in other vertebrates, with well conserved characteristic functional domains and catalytic residues. SaCTSDb showed phylogenetic, sequence and tissue distribution differences with respect to its paralogue SaCTSDa, previously identified in the ovary. Expression of gilthead sea bream cathepsins (B, L, Da, Db) and UbP members (N3, Ub, MuRF1 and MAFbx) in fast skeletal muscle was determined at three different life-history stages and in response to fasting and re-feeding in juveniles. Most of the proteolytic genes analysed were significantly up-regulated during fasting, and down-regulated with re-feeding and, between the fingerling (15 g) and juvenile/adult stages (~50/500 g), consistent with a decrease in muscle proteolysis in both later contexts. In contrast, SaCTSDa and SaMuRF1 expression was relatively stable with ontogeny and SaUb had higher expression in fingerlings and adults than juveniles.
conclusionsThe data obtained in the present study suggest that cathepsins and UbP genes in gilthead sea bream are co-ordinately regulated during ontogeny to control muscle growth, and indicate that feeding regimes can modulate their expression, providing a potential dietary method of influencing post-mortem fillet tenderisation, and hence, product quality.
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