ArticleReproductive biology and endocrinology : RB&E2015
Beneficial effects of relaxin on motility characteristics of stored boar spermatozoa.
Article in Reproductive biology and endocrinology : RB&E, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 17 citations in OpenAlex.
- A role for mutations inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The molecular phenotype of kisspeptin neurons in the medial amygdala of female mice.Frontiers in endocrinology · 2023Article
- Boar sperm quality and oxidative status as affected by rosmarinic acid at 17 °C.Tropical animal health and production · 2020Article
- Nanotechnology-based approach for safer enrichment of semen with best spermatozoa.Journal of animal science and biotechnology · 2019Article
- Effects of human relaxin-2 (serelaxin) on hypoxic pulmonary vasoconstriction during acute hypoxia in a sheep model.Hypoxia (Auckland, N.Z.) · 2018Article
- Insulin-like peptide 3 expressed in the silkworm possesses intrinsic disulfide bonds and full biological activity.Scientific reports · 2017Article
- Relaxin-like peptides in male reproduction - a human perspective.British journal of pharmacology · 2017Review
- Expression, Localization of SUMO-1, and Analyses of Potential SUMOylated Proteins inFrontiers in physiology · 2017Article
- Self-illuminating quantum dots for non-invasive bioluminescence imaging of mammalian gametes.Journal of nanobiotechnology · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRelaxin is detected in seminal plasma of many species and its association with sperm motility may be beneficial in some aspects of assisted reproduction. Here, we immunolocalized relaxin receptors and investigated the effects of exogenous relaxin on motility characteristics, viability, and cAMP content of boar spermatozoa after storage.
methodsCommercial doses of boar semen were obtained on the collection day (Day 0) and kept in shipping containers at room temperature for up to 4 days (Day 4). On Day 0, spermatozoa were fixed for immunofluorescence detection of relaxin receptors RXFP1 and RXFP2 (Experiment 1). Semen aliquots were taken from the same dose at Day 0, Day 1, and Day 2 (Experiment 2a), and Day 2 and Day 4 (Experiment 2b) for analyses. Alive spermatozoa were purified and incubated (1 h-37°C) with 0, 50, or 100 ng relaxin/ml (Experiment 2a) and 0, 100, or 500 ng relaxin/ml (Experiment 2b). Afterward, aliquots of each treatment group were subjected to motility (Experiments 2), viability (Experiment 3) analyses, and cAMP quantification (Experiment 4). Data (3-4 independent replicates) were statistically analyzed (ANOVA followed by pairwise comparisons) and p values less or equal to 0.05 was set for significant difference.
resultsBoth RXFP1 and RXFP2 receptors were immunolocalized on the entire spermatozoon. Relaxin concentration of 100 ng/ml significantly improved the proportions of motile, progressive, and rapid spermatozoa up to Day 2. Only 500 ng relaxin/ml provided beneficial effects on Day 4. The viability of spermatozoa was not affected by relaxin (100 ng/ml) during storage, but the extent of mitochondria membrane damages was significantly decreased. Furthermore, relaxin did not affect the cAMP contents of spermatozoa during storage, in our conditions.
conclusionsRelaxin could be a valuable motility booster of stored- or aged-spermatozoa for assisted reproduction techniques. However, the related-intracellular signaling cascades of relaxin in boar spermatozoa remain undetermined.
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