ArticlePloS one2015
The large ribosomal subunit protein L9 enables the growth of EF-P deficient cells and enhances small subunit maturation.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Translation factors that keep the ribosome Pro-active.Microbiology and molecular biology reviews : MMBR · 2026Review
- Dual roles of genes required for intrinsic resistance to clarithromycin in evasion of killing by serum complement inInfection and immunity · 2026Article
- Altering translation allows E. coli to overcome G-quadruplex stabilizers.Nucleic acids research · 2025Article
- Identifying immunoreactive proteins in brucellin for enhanced brucellosis diagnosis: a proteomic approach.Frontiers in microbiology · 2025Article
- The intrinsic macrolide resistome ofAntimicrobial agents and chemotherapy · 2024Article
- The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services.Nature communications · 2023Article
- Review
- Translational regulation of environmental adaptation in bacteria.The Journal of biological chemistry · 2020Review
- Ribosome collisions alter frameshifting at translational reprogramming motifs in bacterial mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Culmination of a half-century quest reveals insight into mutant tRNA-mediated frameshifting after tRNA departure from the decoding site.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Chlorosis as a Developmental Program in Cyanobacteria: The Proteomic Fundament for Survival and Awakening.Molecular & cellular proteomics : MCP · 2018Article
- Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.Nucleic acids research · 2017Article
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- A robust platform for chemical genomics in bacterial systems.Molecular biology of the cell · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The loss of the large ribosomal protein L9 causes a reduction in translation fidelity by an unknown mechanism. To identify pathways affected by L9, we identified mutants of E. coli that require L9 for fitness. In a prior study, we characterized L9-dependent mutations in the essential GTPase Der (EngA). Here, we describe a second class of L9-dependent mutations that either compromise or inactivate elongation factor P (EF-P, eIF5A in eukaryotes). Without L9, Δefp cells are practically inviable. Cell fractionation studies revealed that, in both the Der and EF-P mutant cases, L9's activity reduces immature 16S rRNA in 30S particles and partially restores the abundance of monosomes. Inspired by these findings, we discovered that L9 also enhances 16S maturation in wild-type cells. Surprisingly, although the amount of immature 16S in 30S particles was found to be elevated in ΔrplI cells, the amount in polysomes was low and inversely correlated with the immature 16S abundance. These findings provide an explanation for the observed fitness increases afforded by L9 in these mutants and reveal particular physiological conditions in which L9 becomes critical. Additionally, L9 may affect the partitioning of small subunits containing immature 16S rRNA.
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