Evidence map›Paper›PMID 25879201›Full record

ArticleBMC research notes2015

Inactivation of non-enveloped virus by 1,5 iodonaphthyl azide.

Paridhi Gupta, Anuj Sharma, Viard Mathias, Yossef Raviv, Robert Blumenthal, Radha K Maheshwari

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Paridhi GuptaDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. paridhi.gupta.ctr@usuhs.edu.
Anuj SharmaDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. anuj.sharma.ctr@usuhs.edu.
Viard MathiasBasic Science Program, Leidos Biomedical Research, Inc., NCI Center for Cancer Research, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. viardm@mail.nih.gov.
Yossef RavivBasic Science Program, Leidos Biomedical Research, Inc., NCI Center for Cancer Research, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. ravivyossi@gmail.com.
Robert BlumenthalChemical Biology Lab, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA. blumenthalr@mail.nih.gov.
Radha K MaheshwariDepartment of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA. radha.maheshwari@usuhs.edu.
Uniformed Services University of the Health Sciences · USLeidos (United States) · USCenter for Cancer Research · US

Funding

CCR NIH HHS HHSN261200800001CIntramural NIH HHSNCI NIH HHS HHSN261200800001E
6 · The paper itself

Abstract

backgroundA photoactive hydrophobic agent 1,5-iodonaphthyl-azide (INA), has been previously shown to completely inactivate the enveloped viruses. INA sequesters into the lipid bilayer of the virus envelope and upon UV-irradiation bind to the hydrophobic domains of the envelope glycoproteins. In our earlier study, we have shown that the Venezuelan equine encephalitis virus (VEEV) genomic RNA was also inactivated during the inactivation of the virus with INA.

findingsIn the present study, we evaluated if the RNA inactivation property of INA can be used to inactivate non-enveloped RNA viruses. Encephalomyocarditis virus (EMCV) was used as a model non-enveloped virus. Treatment with INA followed by UV-irradiation resulted in complete inactivation of EMCV. RNA isolated from INA-inactivated EMCV was non-infectious and INA was found to be associated with the viral RNA genome. INA-inactivated EMCV induced robust total antibody response. However binding capacity of INA-inactivated EMCV to neutralizing antibody was inhibited.

conclusionThis is the first study to show that INA can completely inactivate non-enveloped virus. Our results suggest that the amino acid composition of the neutralizing epitope may interfere with the protective antibody response generated by the INA-inactivated non-enveloped virus.

Indexed as

AnimalsAntibodies, NeutralizingAntibodies, ViralAntiviral AgentsAzidesCardiovirus InfectionsCell LineEncephalomyocarditis virusEpitopesFibroblastsImmunizationMicePhotochemical ProcessesRNA, ViralUltraviolet RaysVaccines, AttenuatedAntibodies, NeutralizingAntibodies, ViralAntiviral AgentsAzidesEpitopesiodonaphthylazideRNA, ViralVaccines, AttenuatedViral Vaccines

Identifiers

PMID25879201
PMCPMC4339248
OpenAlexW2077907650

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.