ArticleBioMed research international2015
The rapid inactivation of porcine skin by applying high hydrostatic pressure without damaging the extracellular matrix.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Biomechanical comparison of high hydrostatic pressure versus liquid nitrogen treatment for devitalized bone grafts: a fresh porcine model study.BMC musculoskeletal disorders · 2026Article
- A high-hydrostatic pressure device for nevus tissue inactivation and dermal regeneration for reconstructing skin defects after giant congenital melanocytic nevus excision: a clinical trial.Regenerative therapy · 2023Article
- Development of a novel regenerative therapy for malignant bone tumors using an autograft containing tumor inactivated by high hydrostatic pressurization (HHP).Regenerative therapy · 2023Article
- Application of decellularized vascular matrix in small-diameter vascular grafts.Frontiers in bioengineering and biotechnology · 2022Review
- Long-term observation of airway reconstruction using decellularized tracheal allografts in micro-miniature pigs at growing stage.Regenerative therapy · 2020Article
- Hydrostatic pressure can induce apoptosis of the skin.Scientific reports · 2020Article
- Simple and efficient method for consecutive inactivation-cryopreservation of porcine skin grafts.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2020Article
- High Hydrostatic Pressure Therapy Annihilates Squamous Cell Carcinoma in a Murine Model.BioMed research international · 2020Article
- Exploration of the Pressurization Condition for Killing Human Skin Cells and Skin Tumor Cells by High Hydrostatic Pressure.BioMed research international · 2020Article
- Article
- Melanin pigments in the melanocytic nevus regress spontaneously after inactivation by high hydrostatic pressure.PloS one · 2017Article
- An Exploratory Clinical Trial of a Novel Treatment for Giant Congenital Melanocytic Nevi Combining Inactivated Autologous Nevus Tissue by High Hydrostatic Pressure and a Cultured Epidermal Autograft: Study Protocol.JMIR research protocols · 2016Article
- The Alteration of the Epidermal Basement Membrane Complex of Human Nevus Tissue and Keratinocyte Attachment after High Hydrostatic Pressurization.BioMed research international · 2016Article
- Preparation of Inactivated Human Skin Using High Hydrostatic Pressurization for Full-Thickness Skin Reconstruction.PloS one · 2015Article
- Comprehensive characterization of cell and tissue responses toward high hydrostatic pressure treatment: Molecular feedback and structural integrity in bone graft processing.Journal of tissue engineeringArticle
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that high hydrostatic pressure (HHP) of 200 MPa for 10 minutes could induce cell killing. In this study, we explored whether HHP at 200 MPa or HHP at lower pressure, in combination with hyposmotic distilled water (DW), could inactivate the skin, as well as cultured cells. We investigated the inactivation of porcine skin samples 4 mm in diameter. They were immersed in either a normal saline solution (NSS) or DW, and then were pressurized at 100 and 200 MPa for 5, 10, 30, or 60 min. Next, we explored the inactivation of specimens punched out from the pressurized skin 10×2 cm in size. The viability was evaluated using a WST-8 assay and an outgrowth culture. The histology of specimens was analyzed histologically. The mitochondrial activity was inactivated after the pressurization at 200 MPa in both experiments, and no outgrowth was observed after the pressurization at 200 MPa. The arrangement and proportion of the dermal collagen fibers or the elastin fibers were not adversely affected after the pressurization at 200 MPa for up to 60 minutes. This study showed that a HHP at 200 MPa for 10 min could inactivate the skin without damaging the dermal matrix.
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