ArticleAdvanced biomedical research2015
Easy method for production of a home-made DNA ladder in every laboratory.
Article in Advanced biomedical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- A flexible, cost- and time-saving method for in-house laboratory production of DNA markers.Biotechnology letters · 2025Article
- pHAPE: a plasmid for production of DNA size marker ladders for gel electrophoresis.Biology methods & protocols · 2023Article
- The pPSU Plasmids for Generating DNA Molecular Weight Markers.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMolecular DNA markers are one of the essential tools in molecular biology labs with varied applications. In the present study, we suggest an efficient and available strategy to produce molecular size marker in routine laboratories. MATERIALS AND
methodsTo achieve the desired sizes of DNA fragments, we recruited PCR and bioinformatics techniques to synthesize 14 DNA fragments ranging from 100 to 3000 bp.
resultsHolistic analysis of different parameters in primers design resulted in amplification of fragments in just one PCR program without any by-product and purification step. Our applied method enables researchers to modify amplified DNA fragments by wide range of chemical modifications toward varied applications.
conclusionMethod of home-made DNA ladder production by available ingredients and routine techniques reported in this study can be used in common laboratories for different applications.
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Registered trials
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