ArticleJournal of clinical microbiology2015
Molecular Assay for Detection of Genetic Markers Associated with Decreased Susceptibility to Cephalosporins in Neisseria gonorrhoeae.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Development and evaluation of dried urine strip for genital chlamydia and gonorrhea testing.Journal of clinical microbiology · 2026Article
- World Health Organization-Recommended Periodic Presumptive Treatment Versus Doxycycline Post-Exposure Prophylaxis for Sexually Transmitted Infection Control Among Men Who Have Sex With Men in Kenya: Protocol for a Randomized Controlled Trial.JMIR research protocols · 2026Article
- Use of genome sequencing to resolve differences in gradient diffusion and agar dilution antimicrobial susceptibility testing performance ofJournal of clinical microbiology · 2023Article
- Resistance-Guided Therapy for Neisseria gonorrhoeae.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Loop-Mediated Isothermal Amplification Assay for Identifying Neisseria gonorrhoeae NonmosaicMicrobiology spectrum · 2022Article
- Reliability of Genetic Alterations in Predicting Ceftriaxone Resistance in Neisseria gonorrhoeae Globally.Microbiology spectrum · 2022Article
- Evaluation of the Hologic Aptima Combo 2 Assay for Detection of Neisseria gonorrhoeae from Joint Fluid Specimens.Journal of clinical microbiology · 2022Article
- High-Resolution Melting Analysis to Detect Antimicrobial Resistance Determinants in South AfricanInternational journal of microbiology · 2022Article
- Review
- Establishing Novel Molecular Algorithms to Predict Decreased Susceptibility to Ceftriaxone in Neisseria gonorrhoeae Strains.The Journal of infectious diseases · 2021Review
- Evaluating the generalizability of a multiplex real-time PCR assay for predicting decreased susceptibility to ceftriaxone in a global set of Neisseria gonorrhoeae sequences.The Journal of antimicrobial chemotherapy · 2021Article
- Equations To Predict Antimicrobial MICs in Neisseria gonorrhoeae Using Molecular Antimicrobial Resistance Determinants.Antimicrobial agents and chemotherapy · 2020Article
- The Laboratory Diagnosis ofPathogens (Basel, Switzerland) · 2020Review
- Genetic Characterization and Enhanced Surveillance of Ceftriaxone-Resistant Neisseria gonorrhoeae Strain, Alberta, Canada, 2018.Emerging infectious diseases · 2019Article
- Addressing the rising rates of gonorrhea and drug-resistant gonorrhea: There is no time like the present.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2019Article
- Multidrug-resistant and extensively drug-resistantCanada communicable disease report = Releve des maladies transmissibles au Canada · 2019Article
- Molecular screening for Neisseria gonorrhoeae antimicrobial resistance markers in Nigerian men who have sex with men and transgender women.International journal of STD & AIDS · 2018Article
- Considering the Potential Application of Whole Genome Sequencing to Gonorrhea Prevention and Control.Sexually transmitted diseases · 2018Article
- Molecular Characterization of Markers Associated With Antimicrobial Resistance in Neisseria gonorrhoeae Identified From Residual Clinical Samples.Sexually transmitted diseases · 2018Article
- Gonorrhea in Canada, 2010-2015.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2018Article
Corrections and comments
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Authors and funding
14 authors at 8 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay for surveillance use to detect mutations in genes associated with decreased susceptibility to cephalosporins that can be applied to both culture isolates and clinical samples. Real-time PCR assays were developed to detect single nucleotide polymorphisms (SNPs) in ponA, mtrR, penA, porB, and one N. gonorrhoeae-specific marker (porA). We tested the real-time PCR assay with 252 gonococcal isolates, 50 nongonococcal isolates, 24 N. gonorrhoeae-negative NAAT specimens, and 34 N. gonorrhoeae-positive NAAT specimens. Twenty-four of the N. gonorrhoeae-positive NAAT specimens had matched culture isolates. Assay results were confirmed by comparison with whole-genome sequencing data. For 252 N. gonorrhoeae strains, the agreement between the DNA sequence and real-time PCR was 100% for porA, ponA, and penA, 99.6% for mtrR, and 95.2% for porB. The presence of ≥2 SNPs correlated with decreased susceptibility to ceftriaxone (sensitivities of >98%) and cefixime (sensitivities of >96%). Of 24 NAAT specimens with matched cultures, the agreement between the DNA sequence and real-time PCR was 100% for porB, 95.8% for ponA and mtrR, and 91.7% for penA. We demonstrated the utility of a real-time PCR assay for sensitive detection of known markers for the decreased susceptibility to cephalosporins in N. gonorrhoeae. Preliminary results with clinical NAAT specimens were also promising, as they correlated well with bacterial culture results.
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