ArticleThe Journal of biological chemistry2015
Thioredoxin Selectivity for Thiol-based Redox Regulation of Target Proteins in Chloroplasts.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 118 citations in OpenAlex.
- The thioredoxin system protects PSI from photoinhibition in coordination with PSI cyclic electron transport under fluctuating light conditions.Plant & cell physiology · 2026Article
- Systematic determination of disulfide bond reduction potentials reveals a nonequilibrium redox hierarchy in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Plastidial phosphoglucose isomerase undergoes thioredoxin-mediated redox modification without altering catalytic activity.The Biochemical journal · 2025Article
- Diversity and distribution of thioredoxin family proteins in photosynthetic organisms.Plant & cell physiology · 2025Article
- Functional relationship of atypical thioredoxins with NADPH-thioredoxin reductase C and 2-Cys peroxiredoxins in Arabidopsis chloroplasts.Journal of experimental botany · 2025Article
- Cracking the Code of Oocyte Quality: The Oxidative Stress Link to IVF Success.International journal of molecular sciences · 2025Review
- Mechanism and application of thiol-disulfide redox biosensors with a fluorescence-lifetime readout.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Plastidial thioredoxin-like proteins are essential for normal embryogenesis and seed development in Arabidopsis thaliana.Journal of plant research · 2025Article
- Cysteine residues contribute to the regulation of Arabidopsis state transition 7 kinase.FEBS letters · 2025Article
- Functional recoding ofFrontiers in plant science · 2025Article
- Chilling- and dark-regulated photoprotection in Miscanthus, an economically important CCommunications biology · 2024Article
- The impact of light and thioredoxins on the plant thiol-disulfide proteome.Plant physiology · 2024Article
- Divergent Protein Redox Dynamics and Their Relationship with Electron Transport Efficiency during Photosynthesis Induction.Plant & cell physiology · 2024Article
- Overexpression of thioredoxin-like protein ACHT2 leads to negative feedback control of photosynthesis in Arabidopsis thaliana.Journal of plant research · 2024Article
- NTRC and thioredoxins m1/m2 underpin the light acclimation of plants on proteome and metabolome levels.Plant physiology · 2024Article
- Members of theInternational journal of molecular sciences · 2024Article
- x- and y-type thioredoxins maintain redox homeostasis on photosystem I acceptor side under fluctuating light.Plant physiology · 2023Article
- Current Insights into the Redox Regulation Network in Plant Chloroplasts.Plant & cell physiology · 2023Review
- Article
- The Functional Relationship between NADPH Thioredoxin Reductase C, 2-Cys Peroxiredoxins, andAntioxidants (Basel, Switzerland) · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Redox regulation based on the thioredoxin (Trx) system is believed to ensure light-responsive control of various functions in chloroplasts. Five Trx subtypes have been reported to reside in chloroplasts, but their functional diversity in the redox regulation of Trx target proteins remains poorly clarified. To directly address this issue, we studied the Trx-dependent redox shifts of several chloroplast thiol-modulated enzymes in vitro and in vivo. In vitro assays using a series of Arabidopsis recombinant proteins provided new insights into Trx selectivity for the redox regulation as well as the underpinning for previous suggestions. Most notably, by combining the discrimination of thiol status with mass spectrometry and activity measurement, we identified an uncharacterized aspect of the reductive activation of NADP-malate dehydrogenase; two redox-active Cys pairs harbored in this enzyme were reduced via distinct utilization of Trxs even within a single polypeptide. In our in vitro assays, Trx-f was effective in reducing all thiol-modulated enzymes analyzed here. We then investigated the in vivo physiological relevance of these in vitro findings, using Arabidopsis wild-type and Trx-f-deficient plants. Photoreduction of fructose-1,6-bisphosphatase was partially impaired in Trx-f-deficient plants, but the global impact of Trx-f deficiency on the redox behaviors of thiol-modulated enzymes was not as striking as expected from the in vitro data. Our results provide support for the in vivo functionality of the Trx system and also highlight the complexity and plasticity of the chloroplast redox network.
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