ArticleParasitology research2015
Intracellular amastigote replication may not be required for successful in vitro selection of miltefosine resistance in Leishmania infantum.
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Identification of Resistance Determinants for a Promising Antileishmanial Oxaborole Series.Microorganisms · 2021Article
- Antileishmanial Aminopyrazoles: Studies into Mechanisms and Stability of Experimental Drug Resistance.Antimicrobial agents and chemotherapy · 2020Article
- Phenotypic adaptations of Leishmania donovani to recurrent miltefosine exposure and impact on sand fly infection.Parasites & vectors · 2020Article
- Interferon Alpha Favors Macrophage Infection by VisceralFrontiers in immunology · 2020Article
- Impaired development of a miltefosine-resistant Leishmania infantum strain in the sand fly vectors Phlebotomus perniciosus and Lutzomyia longipalpis.International journal for parasitology. Drugs and drug resistance · 2019Article
- In-depth comparison of cell-based methodological approaches to determine drug susceptibility of visceral Leishmania isolates.PLoS neglected tropical diseases · 2019Article
- Immunosuppression of Syrian golden hamsters accelerates relapse but not the emergence of resistance in Leishmania infantum following recurrent miltefosine pressure.International journal for parasitology. Drugs and drug resistance · 2019Article
- Impact of primary mouse macrophage cell types on Leishmania infection and in vitro drug susceptibility.Parasitology research · 2018Article
- Genomic and transcriptomic alterations in Leishmania donovani lines experimentally resistant to antileishmanial drugs.International journal for parasitology. Drugs and drug resistance · 2018Article
- Evaluating drug resistance in visceral leishmaniasis: the challenges.Parasitology · 2018Review
- Combined treatment of miltefosine and paromomycin delays the onset of experimental drug resistance in Leishmania infantum.PLoS neglected tropical diseases · 2017Article
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Authors and funding
7 authors.
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Abstract
Although miltefosine (MIL) has only recently been positioned as a first-line therapeutic option for visceral leishmaniasis, field reports note an increasing trend in treatment failures. Study of laboratory selected MIL-resistant strains is needed in the absence of confirmed resistant clinical isolates. In contrast to promastigotes, experimental in vitro selection of MIL-resistance on intracellular amastigotes has not yet been documented. This study reports for the first time the selection of MIL-resistance in Leishmania infantum LEM3323, a strain which clearly shows active intracellular replication. Starting from the hypothesis that active multiplication may be essential in the resistance selection process; several other L. infantum strains were evaluated. Although strain LEM5269 showed only marginally lower intracellular multiplication, selection for resistance failed, as was also the case for several other strains showing poor or no intracellular replication. These results suggest that intracellular multiplication may not be an absolute prerequisite for the outcome of experimental in vitro MIL-resistance selection in clinical field isolates.
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