ArticlePloS one2015
Molecular characterization of a multidrug resistance IncF plasmid from the globally disseminated Escherichia coli ST131 clone.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Meta-analysis of Pandemic Escherichia coli ST131 Plasmidome Proves Restricted Plasmid-clade Associations.Scientific reports · 2020Pooled it
- Applications of transposon-insertion sequencing for understanding bacterial physiology.Microbial genomics · 2026Review
- Genomic Characterization of Selected Multidrug-ResistantAnimals : an open access journal from MDPI · 2026Article
- Emerging Dissemination of blaMicrobiologyOpen · 2026Article
- Whole-genome characterisation of high-risk ST131 and the emerging ST1193 UPEC lineages in Romanian patients with recurrent urinary tract infections.Frontiers in microbiology · 2026Article
- Selective elimination of donor bacteria enables global profiling of plasmid gene expression at early stages of conjugation.Nucleic acids research · 2025Article
- Article
- Plasmid-mediated acquisition and chromosomal integration ofAntimicrobial agents and chemotherapy · 2024Article
- Influence of COAntibiotics (Basel, Switzerland) · 2024Article
- Article
- Short- and Long-Read Sequencing Reveals the Presence and Evolution of an IncF Plasmid HarboringMicrobiology spectrum · 2023Article
- Genetic basis of I-complex plasmid stability and conjugation.PLoS genetics · 2023Article
- Genomic and functional portrait of multidrug-resistant, hydrogen sulfide (HFrontiers in microbiology · 2023Article
- Current Treatment Strategies Against Multidrug-Resistant Bacteria: A Review.Current microbiology · 2022Review
- Genomic Characterization of a UropathogenicFrontiers in microbiology · 2022Article
- Plasmids shape the diverse accessory resistomes ofAccess microbiology · 2021Article
- Mechanisms of Theta Plasmid Replication in Enterobacteria and Implications for Adaptation to Its Host.EcoSal Plus · 2020Review
- F Plasmids Are the Major Carriers of Antibiotic Resistance Genes in Human-Associated Commensal Escherichia coli.mSphere · 2020Article
- Article
- Genomic Surveillance of Ceftriaxone-ResistantFrontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli sequence type 131 (E. coli ST131) is a recently emerged and globally disseminated multidrug resistant clone associated with urinary tract and bloodstream infections. Plasmids represent a major vehicle for the carriage of antibiotic resistance genes in E. coli ST131. In this study, we determined the complete sequence and performed a comprehensive annotation of pEC958, an IncF plasmid from the E. coli ST131 reference strain EC958. Plasmid pEC958 is 135.6 kb in size, harbours two replicons (RepFIA and RepFII) and contains 12 antibiotic resistance genes (including the blaCTX-M-15 gene). We also carried out hyper-saturated transposon mutagenesis and multiplexed transposon directed insertion-site sequencing (TraDIS) to investigate the biology of pEC958. TraDIS data showed that while only the RepFII replicon was required for pEC958 replication, the RepFIA replicon contains genes essential for its partitioning. Thus, our data provides direct evidence that the RepFIA and RepFII replicons in pEC958 cooperate to ensure their stable inheritance. The gene encoding the antitoxin component (ccdA) of the post-segregational killing system CcdAB was also protected from mutagenesis, demonstrating this system is active. Sequence comparison with a global collection of ST131 strains suggest that IncF represents the most common type of plasmid in this clone, and underscores the need to understand its evolution and contribution to the spread of antibiotic resistance genes in E. coli ST131.
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