ArticlePloS one2015
Down-regulation of microRNA-223 promotes degranulation via the PI3K/Akt pathway by targeting IGF-1R in mast cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- SJB2-043, a USP1 Inhibitor, Suppresses A549 Cell Proliferation, Migration, and EMT via Modulation of PI3K/AKT/mTOR, MAPK, and Wnt Signaling Pathways.Current issues in molecular biology · 2025Article
- Characterizing the Inflammatory Microenvironment in K14-HPV16 Transgenic Mice: Mast Cell Infiltration and MicroRNA Expression.Cancers · 2022Article
- Two Sides of the Coin: Mast Cells as a Key Regulator of Allergy and Acute/Chronic Inflammation.Cells · 2021Review
- Epigenetic Changes in Neoplastic Mast Cells and Potential Impact in Mastocytosis.International journal of molecular sciences · 2021Review
- MicroRNA-221 Modulates Airway Remodeling via the PI3K/AKT Pathway in OVA-Induced Chronic Murine Asthma.Frontiers in cell and developmental biology · 2020Article
- MicroRNA Involvement in Allergic and Non-Allergic Mast Cell Activation.International journal of molecular sciences · 2019Review
- miRNA-223 is an anticancer gene in human non-small cell lung cancer through the PI3K/AKT pathway by targeting EGFR.Oncology reports · 2019Article
- Can alternative epitope mapping approaches increase the impact of B-cell epitopes in food allergy diagnostics?Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2019Review
- Coptisine Suppresses Mast Cell Degranulation and Ovalbumin-Induced Allergic Rhinitis.Molecules (Basel, Switzerland) · 2018Article
- Propofol attenuates mast cell degranulation via inhibiting the miR-221/PI3K/Akt/CaExperimental and therapeutic medicine · 2018Article
- MiR-302e attenuates allergic inflammationBioscience reports · 2018Article
- Ion-Exchange Chromatography Coupled With Dynamic Coating Capillary Electrophoresis for Simultaneous Determination of Tropomyosin and Arginine Kinase in Shellfish.Frontiers in chemistry · 2018Article
- MicroRNA-223 inhibits deposition of the extracellular matrix by airway smooth muscle cells through targeting IGF-1R in the PI3K/Akt pathway.American journal of translational research · 2018Article
- Transcriptional determination and functional specificity of myeloid cells: making sense of diversity.Nature reviews. Immunology · 2017Review
- Inhibition of airway inflammation in a cockroach allergen model of asthma by agonists of miRNA-33b.Scientific reports · 2017Article
- Bile duct ligation-induced biliary hyperplasia, hepatic injury, and fibrosis are reduced in mast cell-deficient KitHepatology (Baltimore, Md.) · 2017Article
- Article
- P2Y6 contributes to ovalbumin-induced allergic asthma by enhancing mast cell function in mice.Oncotarget · 2016Article
- miR-223 enhances the sensitivity of non-small cell lung cancer cells to erlotinib by targeting the insulin-like growth factor-1 receptor.International journal of molecular medicine · 2016Article
- Oral squamous cell carcinoma: microRNA expression profiling and integrative analyses for elucidation of tumourigenesis mechanism.Molecular cancer · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMast cells play a central role in allergic and inflammatory disorders by inducing degranulation and inflammatory mediator release. Recent reports have shown that miRNAs play an important role in inflammatory response regulation. Therefore, the role of miR-223 in mast cells was investigated.
methodsThe expression of miR-223 was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) in immunoglobulin E (IgE)-mediated mast cells. After successful miR-223 inhibition by transfection, degranulation was detected in IgE-mediated mast cells. The phosphorylation of IκB-α and Akt were examined using western blotting. NF-κB was tested using electrophoretic mobility shift assay. PI3K-inhibitor (LY294002) was used to investigate whether the PI3K/Akt pathway was essential for mast cell activation. The TargetScan database and a luciferase reporter system were used to identify whether insulin-like growth factor 1 receptor (IGF-1R) is a direct target of miR-223.
resultsMiR-223 expression was up-regulated in IgE-mediated mast cells, whereas its down-regulation promoted mast cell degranulation. Levels of IκB-α and Akt phosphorylation as well as NF-κB were increased in miR-223 inhibitor cells. LY294002 could block the PI3K/Akt signaling pathway and rescue the promotion caused by suppressing miR-223 in mast cells. IGF-1R was identified as a direct target of miR-223.
conclusionsThese findings suggest that down-regulation of miR-223 promotes degranulation via the PI3K/Akt pathway by targeting IGF-1R in mast cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.