ArticlePloS one2015
Comparative transcriptome analysis reveals different molecular mechanisms of Bacillus coagulans 2-6 response to sodium lactate and calcium lactate during lactic acid production.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 46 citations in OpenAlex.
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- Harnessing the dual nature of Bacillus (Weizmannia) coagulans for sustainable production of biomaterials and development of functional food.Microbial biotechnology · 2024Review
- Assimilation of phthalate esters in bacteria.Applied microbiology and biotechnology · 2024Review
- Time-resolved transcriptomic and proteomic profiling ofFrontiers in microbiology · 2023Article
- Identification of a major facilitator superfamily protein that is beneficial to L-lactic acid production by Bacillus coagulans at low pH.BMC microbiology · 2022Article
- Ribosome Profiling Reveals Genome-Wide Cellular Translational Regulation inFoods (Basel, Switzerland) · 2022Article
- Genome-Wide Transcriptomic Analysis ofJournal of microbiology and biotechnology · 2021Article
- DNA Satellites Are Transcribed as Part of the Non-Coding Genome in Eukaryotes and Bacteria.Genes · 2021Article
- Tandem Repeats inInternational journal of molecular sciences · 2021Article
- Transcriptional response of Bacillus megaterium FDU301 to PEG200-mediated arid stress.BMC microbiology · 2020Article
- Unique Features of Tandem Repeats in Bacteria.Journal of bacteriology · 2020Article
- Enhanced ε-Poly-L-Lysine Production by the Synergistic Effect of ε-Poly-L-Lysine Synthetase Overexpression and Citrate inFrontiers in bioengineering and biotechnology · 2020Article
- Genomic and Transcriptomic Insights into Calcium Carbonate Biomineralization by Marine ActinobacteriumFrontiers in microbiology · 2017Article
- Comparison of high-titer lactic acid fermentation from NaOH- and NHScientific reports · 2016Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactate production is enhanced by adding calcium carbonate or sodium hydroxide during fermentation. However, Bacillus coagulans 2-6 can produce more than 180 g/L L-lactic acid when calcium lactate is accumulated, but less than 120 g/L L-lactic acid when sodium lactate is formed. The molecular mechanisms by which B. coagulans responds to calcium lactate and sodium lactate remain unclear. In this study, comparative transcriptomic methods based on high-throughput RNA sequencing were applied to study gene expression changes in B. coagulans 2-6 cultured in non-stress, sodium lactate stress and calcium lactate stress conditions. Gene expression profiling identified 712 and 1213 significantly regulated genes in response to calcium lactate stress and sodium lactate stress, respectively. Gene ontology assignments of the differentially expressed genes were performed. KEGG pathway enrichment analysis revealed that 'ATP-binding cassette transporters' were significantly affected by calcium lactate stress, and 'amino sugar and nucleotide sugar metabolism' was significantly affected by sodium lactate stress. It was also found that lactate fermentation was less affected by calcium lactate stress than by sodium lactate stress. Sodium lactate stress had negative effect on the expression of 'glycolysis/gluconeogenesis' genes but positive effect on the expression of 'citrate cycle (TCA cycle)' genes. However, calcium lactate stress had positive influence on the expression of 'glycolysis/gluconeogenesis' genes and had minor influence on 'citrate cycle (TCA cycle)' genes. Thus, our findings offer new insights into the responses of B. coagulans to different lactate stresses. Notably, our RNA-seq dataset constitute a robust database for investigating the functions of genes induced by lactate stress in the future and identify potential targets for genetic engineering to further improve L-lactic acid production by B. coagulans.
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