ArticlePloS one2015
Comprehensive evaluation of Toxoplasma gondii VEG and Neospora caninum LIV genomes with tachyzoite stage transcriptome and proteome defines novel transcript features.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- First molecular identification and phylogenetic analysis ofJournal of veterinary research · 2026Article
- A novel TaqMan probe-based pentaplex qPCR assay for the simultaneous detection of five pathogenic protozoans.Scientific reports · 2025Article
- Intra-strain genetic heterogeneity in Toxoplasma gondii ME49: Oxford Nanopore long-read sequencing reveals copy number variation in the ROP8-ROP2A locus.BMC genomics · 2025Article
- A bioinformatic approach for the prediction and functional classification of Toxoplasma gondii long non-coding RNAs.Scientific reports · 2024Article
- TgKDAC4: A Unique Deacetylase ofMicroorganisms · 2023Article
- Atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging ofAnalytical science advances · 2022Article
- MicroRNA profiling of Neospora caninum tachyzoites (NC-1) using a high-throughput approach.Parasitology research · 2021Article
- ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.Nature communications · 2021Article
- Noncoding RNAs in Apicomplexan Parasites: An Update.Trends in parasitology · 2020Review
- Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection byPathogens (Basel, Switzerland) · 2020Article
- Analysis of Long Non-Coding RNA inFrontiers in cellular and infection microbiology · 2020Article
- High-Resolution Mapping of Transcription Initiation in the Asexual Stages ofFrontiers in cellular and infection microbiology · 2020Article
- Identification of a PH domain-containing protein which is localized to crystalloid bodies of Plasmodium ookinetes.Malaria journal · 2018Article
- Genome Wide Identification of Mutational Hotspots in the Apicomplexan Parasite Neospora caninum and the Implications for Virulence.Genome biology and evolution · 2018Article
- Everybody needs sphingolipids, right! Mining for new drug targets in protozoan sphingolipid biosynthesis.Parasitology · 2018Review
- Toxoplasma gondii and Neospora caninum induce different host cell responses at proteome-wide phosphorylation events; a step forward for uncovering the biological differences between these closely related parasites.Parasitology research · 2017Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- The genome of the protozoan parasite Cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates.International journal for parasitology · 2017Article
- Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein.Parasites & vectors · 2017Article
- European Nucleotide Archive in 2016.Nucleic acids research · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxoplasma gondii is an important protozoan parasite that infects all warm-blooded animals and causes opportunistic infections in immuno-compromised humans. Its closest relative, Neospora caninum, is an important veterinary pathogen that causes spontaneous abortion in livestock. Comparative genomics of these two closely related coccidians has been of particular interest to identify genes that contribute to varied host cell specificity and disease. Here, we describe a manual evaluation of these genomes based on strand-specific RNA sequencing and shotgun proteomics from the invasive tachyzoite stages of these two parasites. We have corrected predicted structures of over one third of the previously annotated gene models and have annotated untranslated regions (UTRs) in over half of the predicted protein-coding genes. We observe distinctly long UTRs in both the organisms, almost four times longer than other model eukaryotes. We have also identified a putative set of cis-natural antisense transcripts (cis-NATs) and long intergenic non-coding RNAs (lincRNAs). We have significantly improved the annotation quality in these genomes that would serve as a manually curated dataset for Toxoplasma and Neospora research communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.