ArticlePLoS genetics2015
MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback moth.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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Who cites it
105 citing papers in PubMed, 249 citations in OpenAlex.
- Down-regulation of the PxJHE2 gene elevates juvenile hormone titers and compensates for fitness costs of Bt Cry1Ac resistance in Plutella xylostella (L.).Pest management science · 2026Article
- Calcineurin-dependent 20E signaling pathway enhances attacin expression to prime the immunity of Helicoverpa armigera against Bacillus thuringiensis Cry1Ac toxin.PLoS pathogens · 2026Article
- A second locus associated with high-level resistance to Bt Cry1Ac inApplied and environmental microbiology · 2026Article
- Review
- From gatekeeper to target: MAPK cascades as control circuits at the insect-microbe interface.FEMS microbiology reviews · 2026Review
- Gut plasma membrane proteomes of adults and larvae of the European honey bee, Apis mellifera.Scientific reports · 2025Article
- An anti-idiotypic single domain antibody as Cry2Aa toxin mimotope and analysis of its binding region through molecular docking.BMC biotechnology · 2025Article
- Interactions between insecticidal cry toxins and their receptors.Current genetics · 2025Review
- The role of GPI-anchored membrane-bound alkaline phosphatase in the mode of action of Bt Cry1A toxins in the diamondback moth.Fundamental research · 2025Article
- Cry1 resistance in a CRISPR/Cas9-mediated HaCad1 gene knockout strain of the Australian cotton bollworm Helicoverpa armigera conferta (Lepidoptera: Noctuidae).Pest management science · 2025Article
- Effects of Microbes on Insect Host Physiology and Behavior Mediated by the Host Immune System.Insects · 2025Review
- Mismatch between lab-generated and field-evolved resistance to transgenic Bt crops inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Physiological and Molecular Mechanisms of Lepidopteran Insects: Genomic Insights and Applications of Genome Editing for Future Research.International journal of molecular sciences · 2024Review
- A midgut transcriptional regulatory loop favors an insect host to withstand a bacterial pathogen.Innovation (Cambridge (Mass.)) · 2024Article
- Resistance of Lepidopteran Pests toToxins · 2024Review
- A chromosome-level genome assembly of the soybean pod borer: insights into larval transcriptional response to transgenic soybean expressing the pesticidal Cry1Ac protein.BMC genomics · 2024Article
- New Paralogs of theBiomolecules · 2024Article
- RNA mAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Engineering of Bacillus thuringiensis Cry2Ab toxin for improved insecticidal activity.AMB Express · 2024Article
- Insights into the role of non-coding RNAs in the development of insecticide resistance in insects.Frontiers in genetics · 2024Review
45 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously mapped to a multigenic resistance locus (BtR-1). Here, we assembled the 3.15 Mb BtR-1 locus and found high-level resistance to Cry1Ac and Bt biopesticide in four independent P. xylostella strains were all associated with differential expression of a midgut membrane-bound alkaline phosphatase (ALP) outside this locus and a suite of ATP-binding cassette transporter subfamily C (ABCC) genes inside this locus. The interplay between these resistance genes is controlled by a previously uncharacterized trans-regulatory mechanism via the mitogen-activated protein kinase (MAPK) signaling pathway. Molecular, biochemical, and functional analyses have established ALP as a functional Cry1Ac receptor. Phenotypic association experiments revealed that the recessive Cry1Ac resistance was tightly linked to down-regulation of ALP, ABCC2 and ABCC3, whereas it was not linked to up-regulation of ABCC1. Silencing of ABCC2 and ABCC3 in susceptible larvae reduced their susceptibility to Cry1Ac but did not affect the expression of ALP, whereas suppression of MAP4K4, a constitutively transcriptionally-activated MAPK upstream gene within the BtR-1 locus, led to a transient recovery of gene expression thereby restoring the susceptibility in resistant larvae. These results highlight a crucial role for ALP and ABCC genes in field-evolved resistance to Cry1Ac and reveal a novel trans-regulatory signaling mechanism responsible for modulating the expression of these pivotal genes in P. xylostella.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.