ArticlePloS one2015
Parasite load induces progressive spleen architecture breakage and impairs cytokine mRNA expression in Leishmania infantum-naturally infected dogs.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 57 citations in OpenAlex.
- Genetic Regulation of Immune Response in Dogs.Genes · 2025Review
- Splenic macrophage functional profile and its role in the immunopathogenesis of canine visceral leishmaniasis.Frontiers in immunology · 2025Article
- Anatomical Vascular Differences andPathogens (Basel, Switzerland) · 2024Article
- The relationships amongInternational journal for parasitology. Parasites and wildlife · 2024Article
- Refinement of the rKLi8.3-Based Serodiagnostic ELISA Allows Detection of Canine Leishmaniosis in Dogs with Low Antibody Titers.Pathogens (Basel, Switzerland) · 2024Article
- Development of quantitative PCR and digital PCR for the quantification of Leishmania infantum in dogs.Molecular and cellular biochemistry · 2023Article
- Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line.Veterinary research communications · 2023Article
- From Infection to Death: An Overview of the Pathogenesis of Visceral Leishmaniasis.Pathogens (Basel, Switzerland) · 2023Review
- MicroRNA-21 and microRNA-148a affects PTEN, NO and ROS in canine leishmaniasis.Frontiers in genetics · 2023Article
- miR-148a regulation interferes in inflammatory cytokine and parasitic load in canine leishmaniasis.PLoS neglected tropical diseases · 2023Article
- LBMPL Vaccine Therapy Induces Progressive Organization of the Spleen Microarchitecture, Improved Th1 Adaptative Immune Response and Control of Parasitism inPathogens (Basel, Switzerland) · 2022Article
- The outcomes of polyparasitism in stray cats from Brazilian Midwest assessed by epidemiological, hematological and pathological data.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2022Article
- Anti-Pathogens (Basel, Switzerland) · 2021Article
- Epidemiologic, Clinical and Immunological Consequences of Co-Infections during Canine Leishmaniosis.Animals : an open access journal from MDPI · 2021Review
- Hematological Changes in Dogs with Visceral Leishmaniasis Are Associated with Increased IFN-γ and TNF Gene Expression Levels in the Bone Marrow.Microorganisms · 2021Article
- Bidimensional and Contrast-Enhanced Ultrasonography of the Spleen in Dogs Affected by Leishmaniosis.Animals : an open access journal from MDPI · 2021Article
- Effects of Visceralising Leishmania on the Spleen, Liver, and Bone Marrow: A Pathophysiological Perspective.Microorganisms · 2021Review
- Splenic Transcriptional Responses in Severe Visceral Leishmaniasis: Impaired Leukocyte Chemotaxis and Cell Cycle Arrest.Frontiers in immunology · 2021Article
- Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy.Animals : an open access journal from MDPI · 2020Article
- Occurrence of multiple genotype infection caused by Leishmania infantum in naturally infected dogs.PLoS neglected tropical diseases · 2020Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Canine Visceral Leishmaniasis (CVL) shares many aspects with the human disease and dogs are considered the main urban reservoir of L. infantum in zoonotic VL. Infected dogs develop progressive disease with a large clinical spectrum. A complex balance between the parasite and the genetic/immunological background of the host are decisive for infection evolution and clinical outcome. This study comprised 92 Leishmania infected mongrel dogs of various ages from Mato Grosso, Brazil. Spleen samples were collected for determining parasite load, humoral response, cytokine mRNA expression and histopathology alterations. By real-time PCR for the ssrRNA Leishmania gene, two groups were defined; a low (lowP, n = 46) and a high parasite load groups (highP, n = 42). When comparing these groups, results show variable individual humoral immune response with higher specific IgG production in infected animals but with a notable difference in CVL rapid test optical densities (DPP) between highP and lowP groups. Splenic architecture disruption was characterized by disorganization of white pulp, more evident in animals with high parasitism. All cytokine transcripts in spleen were less expressed in highP than lowP groups with a large heterogeneous variation in response. Individual correlation analysis between cytokine expression and parasite load revealed a negative correlation for both pro-inflammatory cytokines: IFNγ, IL-12, IL-6; and anti-inflammatory cytokines: IL-10 and TGFβ. TNF showed the best negative correlation (r2 = 0.231; p<0.001). Herein we describe impairment on mRNA cytokine expression in leishmania infected dogs with high parasite load associated with a structural modification in the splenic lymphoid micro-architecture. We also discuss the possible mechanism responsible for the uncontrolled parasite growth and clinical outcome.
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