ArticlePLoS genetics2015
Asymmetric transcript discovery by RNA-seq in C. elegans blastomeres identifies neg-1, a gene important for anterior morphogenesis.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Landscape and regulation of mRNA translation in the early C. elegans embryo.Cell reports · 2025Article
- Landscape and regulation of mRNA translation in the earlybioRxiv : the preprint server for biology · 2025Article
- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- Live cell imaging of β-tubulin mRNA reveals spatiotemporal expression dynamics in the filamentous fungus Aspergillus oryzae.Scientific reports · 2024Article
- Analysis of developmental gene expression using smFISH andbioRxiv : the preprint server for biology · 2024Article
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- The ERM-1 membrane-binding domain directs erm-1 mRNA localization to the plasma membrane in the C. elegans embryo.Development (Cambridge, England) · 2022Article
- Hand Dissection of Caenorhabditis elegans Intestines.Journal of visualized experiments : JoVE · 2022Article
- A primer for generating and using transcriptome data and gene sets.Development (Cambridge, England) · 2020Review
- mRNA localization is linked to translation regulation in theDevelopment (Cambridge, England) · 2020Article
- Exploratory analysis of transposable elements expression in the C. elegans early embryo.BMC bioinformatics · 2019Article
- A new layer of rRNA regulation by small interference RNAs and the nuclear RNAi pathway.RNA biology · 2017Review
- Localization ofmicroPublication biology · 2017Article
- A Single-Cell Biochemistry Approach Reveals PAR Complex Dynamics during Cell Polarization.Developmental cell · 2017Article
- Article
- A Transcriptional Lineage of the Early C. elegans Embryo.Developmental cell · 2016Article
- Tissue homogeneity requires inhibition of unequal gene silencing during development.The Journal of cell biology · 2016Article
- Suppression of transcriptional drift extends C. elegans lifespan by postponing the onset of mortality.eLife · 2015Article
- POS-1 Promotes Endo-mesoderm Development by Inhibiting the Cytoplasmic Polyadenylation of neg-1 mRNA.Developmental cell · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
After fertilization but prior to the onset of zygotic transcription, the C. elegans zygote cleaves asymmetrically to create the anterior AB and posterior P1 blastomeres, each of which goes on to generate distinct cell lineages. To understand how patterns of RNA inheritance and abundance arise after this first asymmetric cell division, we pooled hand-dissected AB and P1 blastomeres and performed RNA-seq. Our approach identified over 200 asymmetrically abundant mRNA transcripts. We confirmed symmetric or asymmetric abundance patterns for a subset of these transcripts using smFISH. smFISH also revealed heterogeneous subcellular patterning of the P1-enriched transcripts chs-1 and bpl-1. We screened transcripts enriched in a given blastomere for embryonic defects using RNAi. The gene neg-1 (F32D1.6) encoded an AB-enriched (anterior) transcript and was required for proper morphology of anterior tissues. In addition, analysis of the asymmetric transcripts yielded clues regarding the post-transcriptional mechanisms that control cellular mRNA abundance during asymmetric cell divisions, which are common in developing organisms.
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