ArticlePloS one2015
Genome-wide analysis of in vivo binding of the master regulator DasR in Streptomyces coelicolor identifies novel non-canonical targets.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
- Expanding perspectives on specialized metabolism in Streptomyces bacteria.Essays in biochemistry · 2026Review
- γ-Butyrolactone-induced coelimycin synthesis inhibits AtrA-dependent actinorhodin overproduction inmBio · 2026Article
- Lon protease reprograms cellular physiology ofFrontiers in microbiology · 2026Article
- The novel GlcNAc 6-phosphate dehydratase NagS governs a metabolic checkpoint that controls nutrient signaling in Streptomyces.PLoS biology · 2025Article
- Enhanced prediction of expression control in bacterial biosynthetic gene clusters via genomic and functional data integration.Microbial genomics · 2025Article
- Optimized production of concanamycins using a rational metabolic engineering strategy.Metabolic engineering · 2025Article
- Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor.Microbial cell factories · 2024Article
- Impact of the Deletion of Genes of the Nitrogen Metabolism on Triacylglycerol, Cardiolipin and Actinorhodin Biosynthesis inMicroorganisms · 2024Article
- Effects of the pleiotropic regulator DasR on lincomycin production in Streptomyces lincolnensis.Applied microbiology and biotechnology · 2024Article
- Harnessing regulatory networks in Actinobacteria for natural product discovery.Journal of industrial microbiology & biotechnology · 2024Review
- Systems-wide analysis of the ROK-family regulatory geneApplied and environmental microbiology · 2023Article
- Carbon catabolite regulation of secondary metabolite formation, an old but not well-established regulatory system.Microbial biotechnology · 2022Review
- A DasA family sugar binding protein Ste2 links nutrient and oxidative stress to exopolysaccharides production in Streptomyces sp. 139.BMC microbiology · 2022Article
- The virulome ofMicrobial genomics · 2022Article
- Ecology and genomics of Actinobacteria: new concepts for natural product discovery.Nature reviews. Microbiology · 2020Review
- Enhancement of bleomycin production in Streptomyces verticillus through global metabolic regulation of N-acetylglucosamine and assisted metabolic profiling analysis.Microbial cell factories · 2020Article
- Multi-level regulation of coelimycin synthesis in Streptomyces coelicolor A3(2).Applied microbiology and biotechnology · 2019Review
- Article
- Discovery of novel glycerolated quinazolinones from Streptomyces sp. MBT27.Journal of industrial microbiology & biotechnology · 2019Article
- sRNA scr5239 Involved in Feedback Loop Regulation ofFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Streptomycetes produce a wealth of natural products, including over half of all known antibiotics. It was previously demonstrated that N-acetylglucosamine and secondary metabolism are closely entwined in streptomycetes. Here we show that DNA recognition by the N-acetylglucosamine-responsive regulator DasR is growth-phase dependent, and that DasR can bind to sites in the S. coelicolor genome that have no obvious resemblance to previously identified DasR-responsive elements. Thus, the regulon of DasR extends well beyond what was previously predicted and includes a large number of genes with functions far removed from N-acetylglucosamine metabolism, such as genes for small RNAs and DNA transposases. Conversely, the DasR regulon during vegetative growth largely correlates to the presence of canonical DasR-responsive elements. The changes in DasR binding in vivo following N-acetylglucosamine induction were studied in detail and a possible molecular mechanism by which the influence of DasR is extended is discussed. Discussion of DasR binding was further informed by a parallel transcriptome analysis of the respective cultures. Evidence is provided that DasR binds directly to the promoters of all genes encoding pathway-specific regulators of antibiotic production in S. coelicolor, thereby providing an exquisitely simple link between nutritional control and secondary metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.