ArticleMolecules (Basel, Switzerland)2015
Employing response surface methodology for the optimization of ultrasound assisted extraction of lutein and β-carotene from spinach.
Article in Molecules (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
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- Recent advancement in ultrasound-assisted novel technologies for the extraction of bioactive compounds from herbal plants: a review.Food science and biotechnology · 2023Review
- In Vitro Bioactivities of Food Grade Extracts from Yarrow (Achillea millefolium L.) and Stinging Nettle (Urtica dioica L.) Leaves.Plant foods for human nutrition (Dordrecht, Netherlands) · 2023Article
- Beta-carotene and its protective effect on gastric cancer.World journal of clinical cases · 2021Review
- Recovery of β-carotene from pumpkin using switchable natural deep eutectic solvents.Ultrasonics sonochemistry · 2021Article
- Optimization of Lutein Recovery fromBiomolecules · 2021Article
- Kiwi fruit residues from industry processing: study for a maximum phenolic recovery yield.Journal of food science and technology · 2020Article
- Optimization of Ultrasonicated Kaempferol Extraction fromFoods (Basel, Switzerland) · 2020Article
- Comparison of Antioxidant Activities of Different Grape Varieties.Molecules (Basel, Switzerland) · 2018Article
- Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage.Molecules (Basel, Switzerland) · 2018Article
- Exploring the Valuable Carotenoids for the Large-Scale Production by Marine Microorganisms.Marine drugs · 2018Review
- Optimization of Production Conditions for Protoplasts and Polyethylene Glycol-Mediated Transformation ofMolecules (Basel, Switzerland) · 2018Article
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- Optimizing the Maximum Recovery of Dihydromyricetin from Chinese Vine Tea, Ampelopsis grossedentata, Using Response Surface Methodology.Molecules (Basel, Switzerland) · 2017Article
- A Rapid and Simple TLC-Densitometric Method for Assay of Clobetasol Propionate in Topical Solution.Molecules (Basel, Switzerland) · 2017Article
- Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts.Plants (Basel, Switzerland) · 2017Review
- Optimization of Betulinic Acid Extraction from Tecomella undulata Bark Using a Box-Behnken Design and Its Densitometric Validation.Molecules (Basel, Switzerland) · 2016Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extraction of lutein and β-carotene from spinach (Spinacia oleracea L.) leaves is important to the dietary supplement industry. A Box-Behnken design and response surface methodology (RSM) were used to investigate the effect of process variables on the ultrasound-assisted extraction (UAE) of lutein and β-carotene from spinach. Three independent variables, extraction temperature (°C), extraction power (%) and extraction time (min) were studied. Thin-layer chromatography (TLC) followed by UV visualization and densitometry was used as a simple and rapid method for both identification and quantification of lutein and β-carotene during UAE. Methanol extracts of leaves from spinach and authentic standards of lutein and β-carotene were separated by normal-phase TLC with ethyl acetate-acetone (5:4 (v/v)) as the mobile phase. In this study, the combination of TLC, densitometry, and Box-Behnken with RSM methods were effective for the quantitative analysis of lutein and β-carotene from spinach extracts. The resulting quadratic polynomial models for optimizing lutein and β-carotene from spinach had high coefficients of determination of 0.96 and 0.94, respectively. The optimal UAE settings for output of lutein and β-carotene simultaneously from spinach extracts were an extraction temperature of 40 °C, extraction power of 40% (28 W/cm3) and extraction time of 16 min. The identity and purity of each TLC spot was measured using time-of-flight mass spectrometry. Therefore, UAE assisted extraction of carotenes from spinach can provide a source of lutein and β-carotene for the dietary supplement industry.
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