ArticleJournal of functional biomaterials2015
Fibroblastic Transformation of Corneal Keratocytes by Rac Inhibition is Modulated by Extracellular Matrix Structure and Stiffness.
Article in Journal of functional biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- PDGF-Stimulated Corneal Keratocyte Motility and Migration Patterns on Aligned Collagen Fibrils Are Inhibited by Decorin.International journal of molecular sciences · 2026Article
- Emerging in vitro models of corneal stromal fibrosis: Toward human-relevant platforms for anti-scarring therapeutics.Indian journal of ophthalmology · 2026Review
- A Comprehensive Review of the Role of Rho-Kinase Inhibitors in Corneal Diseases.Life (Basel, Switzerland) · 2025Review
- Corneal strain influences keratocyte proliferation and migration through upregulation of ALDH3A1 expression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- ECM stiffness modulates the proliferation but not the motility of primary corneal keratocytes in response to PDGF-BB.Experimental eye research · 2022Article
- Signaling Downstream of Focal Adhesions Regulates Stiffness-Dependent Differences in the TGF-Frontiers in cell and developmental biology · 2022Article
- Unraveling the mechanobiology of cornea: From bench side to the clinic.Frontiers in bioengineering and biotechnology · 2022Review
- Differential effects of Hsp90 inhibition on corneal cells in vitro and in vivo.Experimental eye research · 2021Article
- Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro.Current protocols in cell biology · 2020Article
- ECM Stiffness Controls the Activation and Contractility of Corneal Keratocytes in Response to TGF-β1.Biophysical journal · 2020Article
- Modeling the cornea in 3-dimensions: Current and future perspectives.Experimental eye research · 2020Review
- YAP and TAZ are distinct effectors of corneal myofibroblast transformation.Experimental eye research · 2019Article
- Bio-Orthogonally Crosslinked, In Situ Forming Corneal Stromal Tissue Substitute.Advanced healthcare materials · 2018Article
- Tissue and cellular biomechanics during corneal wound injury and repair.Acta biomaterialia · 2017Article
- Corneal epithelial cells exposed to shear stress show altered cytoskeleton and migratory behaviour.PloS one · 2017Article
- Biomechanical relationships between the corneal endothelium and Descemet's membrane.Experimental eye research · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The goal of this study was to investigate how alterations in extracellular matrix (ECM) biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases. Rabbit corneal keratocytes were plated within standard collagen matrices (2.5 mg/mL) or compressed collagen matrices (~100 mg/mL) and cultured in serum-free media, PDGF BB, IGF, FGF2 or TGFβ1, with or without the Rac1 inhibitor NSC23766 and/or the Rho kinase inhibitor Y-27632. After 1 to 4 days, cells were labeled for F-actin and imaged using confocal microscopy. Keratocytes within standard collagen matrices (which are highly compliant) maintained a dendritic phenotype following culture in serum-free media, PDGF, IGF and FGF, but developed stress fibers in TGFβ1. Keratocytes within compressed collagen (which has high stiffness and low porosity) maintained a dendritic phenotype following culture in serum-free media, PDGF and IGF, but developed stress fibers in both FGF and TGFβ1. The Rac inhibitor had no significant impact on growth factor responses in compliant matrices. Within compressed collagen matrices however, the Rac inhibitor induced fibroblastic transformation in serum-free media, PDGF and IGF. Fibroblast and myofibroblast transformation was blocked by Rho kinase inhibition. Overall, keratocyte growth factor responses appear to be regulated by both the interplay between Rho and Rac signaling, and the structural and mechanical properties of the ECM.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.