ArticlePloS one2015
Bacteriological assessment of healthcare-associated pneumonia using a clone library analysis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Risk factors for drug-resistant pathogens in community-acquired pneumonia: systematic review and meta-analysis.European respiratory review : an official journal of the European Respiratory Society · 2025Pooled it
- Sarcoidosis Associated with Enlarged Mediastinal Lymph Nodes with the Detection of Streptococcus gordonii and Cutibacterium acnes Using a Clone Library Method.Internal medicine (Tokyo, Japan) · 2024Article
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- Antimicrobial peptides: Defending the mucosal epithelial barrier.Frontiers in oral health · 2022Review
- Sepsis and the Human Microbiome. Just Another Kind of Organ Failure? A Review.Journal of clinical medicine · 2021Review
- Refinement of microbiota analysis of specimens from patients with respiratory infections using next-generation sequencing.Scientific reports · 2021Article
- Factors associated with pneumococcal vaccine uptake in elderly subjects referred to the respiratory department.Nagoya journal of medical science · 2020Article
- Pathogenic microorganism detection in AIDS patients using bronchoalveolar lavage fluid.International journal of clinical and experimental pathology · 2020Article
- Pulmonary Nocardiosis Caused by Nocardia exalbida in a Patient with Lung Cancer and Radiation Pneumonitis: A Case Report and Literature Review.Internal medicine (Tokyo, Japan) · 2019Review
- Evaluation of the Mann Assessment of Swallowing Ability in Elderly Patients with Pneumonia.Aging and disease · 2017Article
- Bacteriological incidence in pneumonia patients with pulmonary emphysema: a bacterial floral analysis using the 16S ribosomal RNA gene in bronchoalveolar lavage fluid.International journal of chronic obstructive pulmonary disease · 2017Article
- The significance of oral streptococci in patients with pneumonia with risk factors for aspiration: the bacterial floral analysis of 16S ribosomal RNA gene using bronchoalveolar lavage fluid.BMC pulmonary medicine · 2016Article
- Clinical impact of methicillin-resistant staphylococcus aureus on bacterial pneumonia: cultivation and 16S ribosomal RNA gene analysis of bronchoalveolar lavage fluid.BMC infectious diseases · 2016Article
- Dysphagia, dystussia, and aspiration pneumonia in elderly people.Journal of thoracic disease · 2016Article
- The clinical features of respiratory infections caused by the Streptococcus anginosus group.BMC pulmonary medicine · 2015Article
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9 authors.
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Abstract
backgroundThe causative pathogens of healthcare-associated pneumonia (HCAP) remain controversial, and the use of conventional cultivation of sputum samples is occasionally inappropriate due to the potential for oral bacterial contamination. It is also sometimes difficult to determine whether methicillin-resistant Staphylococcus aureus (MRSA) is a true causative pathogen of HCAP.
methodsWe evaluated the bacterial diversity in bronchoalveolar lavage fluid (BALF) using molecular and cultivation methods in 82 HCAP patients. BALF specimens were obtained from the lesions of pneumonia using bronchoscopy. The bacterial flora was analyzed according to the clone library method using amplified fragments of the 16S ribosomal RNA gene with universal primers. In addition, sputum cultures and the above specimens were assessed.
resultsEighty (97.6%) of the 82 BALF samples obtained from the patients with HCAP showed positive polymerase chain reaction results. The predominant phylotypes detected in the BALF in this study included bacteria common in cases of community- and hospital-acquired pneumonia. In addition, the phylotypes of streptococci and anaerobes were detected in 19 (23.2%) and 8 (9.8%) cases, respectively. In particular, phylotypes of streptococci were highly detected among the patients 75 of age or older. Staphylococcus aureus was cultured in 23 (28.0%) cases using conventional cultivation methods and detected in only 6 (7.3%) cases as predominant phylotypes according to the clone library method.
conclusionsThe clone library analysis of BALF in the HCAP patients detected heterogeneous bacteria and a high incidence of streptococci compared with that observed using cultivation methods. In addition, the results of our study may indicate a lower incidence of MRSA than previously expected in HCAP patients.
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