ArticlePloS one2015
The characterization of modified starch branching enzymes: toward the control of starch chain-length distributions.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 35 citations in OpenAlex.
- Trial
- Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin.Foods (Basel, Switzerland) · 2024Article
- Genome Assembly and Pathway Analysis of Edible Mushroom Agrocybe cylindracea.Genomics, proteomics & bioinformatics · 2020Article
- Intra-Sample Heterogeneity of Potato Starch Reveals Fluctuation of Starch-Binding Proteins According to Granule Morphology.Plants (Basel, Switzerland) · 2019Article
- Transcriptome wide identification and characterization of Starch Synthase enzyme in finger millet.Bioinformation · 2018Article
- Association mapping of starch chain length distribution and amylose content in pea (Pisum sativum L.) using carbohydrate metabolism candidate genes.BMC plant biology · 2017Article
- Transcriptome wide identification and characterization of starch branching enzyme in finger millet.Bioinformation · 2017Article
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- Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Starch is a complex branched glucose polymer whose branch molecular weight distribution (the chain-length distribution, CLD) influences nutritionally important properties such as digestion rate. Chain-stopping in starch biosynthesis is by starch branching enzyme (SBE). Site-directed mutagenesis was used to modify SBEIIa from Zea mays (mSBEIIa) to produce mutants, each differing in a single conserved amino-acid residue. Products at different times from in vitro branching were debranched and the time evolution of the CLD measured by size-exclusion chromatography. The results confirm that Tyr352, Glu513, and Ser349 are important for mSBEIIa activity while Arg456 is important for determining the position at which the linear glucan is cut. The mutant mSBEIIa enzymes have different activities and suggest the length of the transferred chain can be varied by mutation. The work shows analysis of the molecular weight distribution can yield information regarding the enzyme branching sites useful for development of plants yielding starch with improved functionality.
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