ArticlePloS one2015
In vivo and in vitro characterization of a Plasmodium liver stage-specific promoter.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The Road toward a Trojan Horse Malaria Vaccine: Chemical Augmentation of Live Sporozoites without Affecting Their Viability.Molecular pharmaceutics · 2026Article
- Same, same but different: Exploring Plasmodium cell division during liver stage development.PLoS pathogens · 2023Review
- Article
- Article
- Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle.Malaria journal · 2021Article
- Definition of constitutive and stage-enriched promoters in the rodent malaria parasite, Plasmodium yoelii.Malaria journal · 2020Article
- Gene knockdown in malaria parasites via non-canonical RNAi.Nucleic acids research · 2020Article
- Transcriptome analysis of Plasmodium berghei during exo-erythrocytic development.Malaria journal · 2019Article
- The s48/45 six-cysteine proteins: mediators of interaction throughout the Plasmodium life cycle.International journal for parasitology · 2017Review
- Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei.Scientific reports · 2017Article
- An ultrasensitive NanoLuc-based luminescence system for monitoring Plasmodium berghei throughout its life cycle.Malaria journal · 2016Article
- Zinc finger nuclease-based double-strand breaks attenuate malaria parasites and reveal rare microhomology-mediated end joining.Genome biology · 2015Article
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Authors and funding
7 authors.
Funding
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Abstract
Little is known about stage-specific gene regulation in Plasmodium parasites, in particular the liver stage of development. We have previously described in the Plasmodium berghei rodent model, a liver stage-specific (lisp2) gene promoter region, in vitro. Using a dual luminescence system, we now confirm the stage specificity of this promoter region also in vivo. Furthermore, by substitution and deletion analyses we have extended our in vitro characterization of important elements within the promoter region. Importantly, the dual luminescence system allows analyzing promoter constructs avoiding mouse-consuming cloning procedures of transgenic parasites. This makes extensive mutation and deletion studies a reasonable approach also in the malaria mouse model. Stage-specific expression constructs and parasite lines are extremely valuable tools for research on Plasmodium liver stage biology. Such reporter lines offer a promising opportunity for assessment of liver stage drugs, characterization of genetically attenuated parasites and liver stage-specific vaccines both in vivo and in vitro, and may be key for the generation of inducible systems.
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